A MARKED GRADATION IN ACTIVE-CENTER PROPERTIES IN THE CYSTEINE PROTEINASES REVEALED BY NEUTRAL AND ANIONIC REACTIVITY PROBES - REACTIVITY CHARACTERISTICS OF THE THIOL-GROUPS OF ACTINIDIN, FICIN, PAPAIN AND PAPAYA PEPTIDASE-A TOWARDS 4,4'-DIPYRIDYL DISULFIDE AND 585'-DITHIOBIS-(2-NITROBENZOATE) DIANION

被引：23

作者：

BROCKLEHURST, K

MUSHIRI, SM

PATEL, G

WILLENBROCK, F

机构：

来源：

BIOCHEMICAL JOURNAL | 1983年 / 209卷 / 03期

关键词：

D O I：

10.1042/bj2090873

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：873 / 879

页数：7

共 25 条

[1] CHARACTERIZATION OF PAPAYA PEPTIDASE-A AS A CYSTEINE PROTEINASE OF CARICA-PAPAYA L WITH ACTIVE-CENTER PROPERTIES THAT DIFFER FROM THOSE OF PAPAIN BY USING 2,2'-DIPYRIDYL DISULFIDE AND 4-CHLORO-7-NITROBENZOFURAZAN AS REACTIVITY PROBES - USE OF 2-PROTONIC-STATE ELECTROPHILES IN THE IDENTIFICATION OF CATALYTIC-SITE THIOL-GROUPS
BAINES, BS
BROCKLEHURST, K
[J]. BIOCHEMICAL JOURNAL, 1982, 205 (01) : 205 - 211
[2] NECESSARY MODIFICATION TO THE PREPARATION OF PAPAIN FROM ANY HIGH-QUALITY LATEX OF CARICA-PAPAYA AND EVIDENCE FOR THE STRUCTURAL INTEGRITY OF THE ENZYME PRODUCED BY TRADITIONAL METHODS
BAINES, BS
BROCKLEHURST, K
[J]. BIOCHEMICAL JOURNAL, 1979, 177 (02) : 541 - 548
[3] STRUCTURE OF ACTINIDIN, AFTER REFINEMENT AT 1.7-A RESOLUTION
BAKER, EN
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1980, 141 (04) : 441 - 484
[4] BOUDART R, 1970, Patent No. 1196760
[5] BOUDART R, 1969, Patent No. 723163
[6] DIFFERENCES IN THE INTERACTIONS OF THE CATALYTIC GROUPS OF THE ACTIVE-CENTERS OF ACTINIDIN AND PAPAIN - RAPID PURIFICATION OF FULLY ACTIVE ACTINIDIN BY COVALENT CHROMATOGRAPHY AND CHARACTERIZATION OF ITS ACTIVE-CENTER BY USE OF 2-PROTONIC-STATE REACTIVITY PROBES
BROCKLEHURST, K
BAINES, BS
MALTHOUSE, JPG
[J]. BIOCHEMICAL JOURNAL, 1981, 197 (03) : 739 - 746
[7] COVALENT CHROMATOGRAPHY - PREPARATION OF FULLY ACTIVE PAPAIN FROM DRIED PAPAYA-LATEX
BROCKLEHURST, K
CARLSSON, J
KIERSTAN, MP
CROOK, EM
[J]. BIOCHEMICAL JOURNAL, 1973, 133 (03) : 573 - 584
[8] BROCKLEHURST K, 1982, METHOD ENZYMOL, V87, P427
[9] REACTIVITIES OF NEUTRAL AND CATIONIC FORMS OF 2,2'-DIPYRIDYL DISULFIDE TOWARDS THIOLATE ANIONS - DETECTION OF DIFFERENCES BETWEEN THE ACTIVE-CENTERS OF ACTINIDIN, PAPAIN AND FICIN BY A 3-PROTONIC-STATE REACTIVITY PROBE
BROCKLEHURST, K
STUCHBURY, T
MALTHOUSE, JPG
[J]. BIOCHEMICAL JOURNAL, 1979, 183 (02) : 233 - 238
[10] REACTIONS OF PAPAIN AND OF LOW-MOLECULAR-WEIGHT THIOLS WITH SOME AROMATIC DISULFIDES - 2,2'-DIPYRIDYL DISULFIDE AS A CONVENIENT ACTIVE-SITE TITRANT FOR PAPAIN EVEN IN PRESENCE OF OTHER THIOLS
BROCKLEHURST, K
LITTLE, G
[J]. BIOCHEMICAL JOURNAL, 1973, 133 (01) : 67 - 80

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