CHARACTERIZATION OF PAPAYA PEPTIDASE-A AS A CYSTEINE PROTEINASE OF CARICA-PAPAYA L WITH ACTIVE-CENTER PROPERTIES THAT DIFFER FROM THOSE OF PAPAIN BY USING 2,2'-DIPYRIDYL DISULFIDE AND 4-CHLORO-7-NITROBENZOFURAZAN AS REACTIVITY PROBES - USE OF 2-PROTONIC-STATE ELECTROPHILES IN THE IDENTIFICATION OF CATALYTIC-SITE THIOL-GROUPS

被引：28

作者：

BAINES, BS ^{[1
]}

BROCKLEHURST, K ^{[1
]}

机构：

[1] UNIV LONDON,ST BARTHOLOMEWS HOSP,COLL MED,DEPT BIOCHEM & CHEM,LONDON EC1M 6BQ,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1982年 / 205卷 / 01期

关键词：

D O I：

10.1042/bj2050205

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：205 / 211

页数：7

共 25 条

[1] MECHANISM OF ACTION OF PAPAIN WITH A SPECIFIC ANILIDE SUBSTRATE
ANGELIDES, KJ
FINK, AL
[J]. BIOCHEMISTRY, 1979, 18 (11) : 2355 - 2363
[2] CRYOENZYMOLOGY OF PAPAIN - REACTION-MECHANISM WITH AN ESTER SUBSTRATE
ANGELIDES, KJ
FINK, AL
[J]. BIOCHEMISTRY, 1978, 17 (13) : 2659 - 2668
[3] MECHANISM OF THIOL PROTEASE CATALYSIS - DETECTION AND STABILIZATION OF A TETRAHEDRAL INTERMEDIATE IN PAPAIN CATALYSIS
ANGELIDES, KJ
FINK, AL
[J]. BIOCHEMISTRY, 1979, 18 (11) : 2363 - 2369
[4] Baines BS, 1982, J PROTEIN CHEM, V1, P119
[5] BOUDART R, 1970, Patent No. 1196760
[6] BOUDART R, 1969, Patent No. 723163
[7] DIFFERENCES IN THE INTERACTIONS OF THE CATALYTIC GROUPS OF THE ACTIVE-CENTERS OF ACTINIDIN AND PAPAIN - RAPID PURIFICATION OF FULLY ACTIVE ACTINIDIN BY COVALENT CHROMATOGRAPHY AND CHARACTERIZATION OF ITS ACTIVE-CENTER BY USE OF 2-PROTONIC-STATE REACTIVITY PROBES
BROCKLEHURST, K
BAINES, BS
MALTHOUSE, JPG
[J]. BIOCHEMICAL JOURNAL, 1981, 197 (03) : 739 - 746
[8] EVIDENCE THAT THE LACK OF HIGH CATALYTIC ACTIVITY OF THIOLSUBTILISIN TOWARDS SPECIFIC SUBSTRATES MAY BE DUE TO AN INAPPROPRIATELY LOCATED PROTON-DISTRIBUTION SYSTEM - DEMONSTRATION OF HIGHLY NUCLEOPHILIC CHARACTER OF THE THIOL-GROUP OF THIOSUBTILISIN IN THE CATALYTICALLY RELEVANT IONIZATION STATE OF THE ACTIVE-CENTER BY USE OF A 2-PROTONIC-STATE REACTIVITY PROBECL
BROCKLEHURST, K
MALTHOUSE, JPG
[J]. BIOCHEMICAL JOURNAL, 1981, 193 (03) : 819 - 823
[9] BROCKLEHURST K, 1982, METHOD ENZYMOL, V87, P427
[10] EVIDENCE THAT BINDING TO THE S-2-SUBSITE OF PAPAIN MAY BE COUPLED WITH CATALYTICALLY RELEVANT STRUCTURAL-CHANGE INVOLVING THE CYSTEINE-25-HISTIDINE-159 DIAD - KINETICS OF THE REACTION OF PAPAIN WITH A 2-PROTONIC-STATE REACTIVITY PROBE CONTAINING A HYDROPHOBIC SIDE-CHAIN
BROCKLEHURST, K
MALTHOUSE, JPG
SHIPTON, M
[J]. BIOCHEMICAL JOURNAL, 1979, 183 (02) : 223 - 231

← 1 2 3 →