EVIDENCE THAT THE LACK OF HIGH CATALYTIC ACTIVITY OF THIOLSUBTILISIN TOWARDS SPECIFIC SUBSTRATES MAY BE DUE TO AN INAPPROPRIATELY LOCATED PROTON-DISTRIBUTION SYSTEM - DEMONSTRATION OF HIGHLY NUCLEOPHILIC CHARACTER OF THE THIOL-GROUP OF THIOSUBTILISIN IN THE CATALYTICALLY RELEVANT IONIZATION STATE OF THE ACTIVE-CENTER BY USE OF A 2-PROTONIC-STATE REACTIVITY PROBECL

被引：21

作者：

BROCKLEHURST, K

MALTHOUSE, JPG

机构：

来源：

BIOCHEMICAL JOURNAL | 1981年 / 193卷 / 03期

关键词：

D O I：

10.1042/bj1930819

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：819 / 823

页数：5

共 38 条

[1] MECHANISM OF ACTION OF PAPAIN WITH A SPECIFIC ANILIDE SUBSTRATE
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[2] CRYOENZYMOLOGY OF PAPAIN - REACTION-MECHANISM WITH AN ESTER SUBSTRATE
ANGELIDES, KJ
FINK, AL
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[3] MECHANISM OF THIOL PROTEASE CATALYSIS - DETECTION AND STABILIZATION OF A TETRAHEDRAL INTERMEDIATE IN PAPAIN CATALYSIS
ANGELIDES, KJ
FINK, AL
[J]. BIOCHEMISTRY, 1979, 18 (11) : 2363 - 2369
[4] IMPORTANCE OF CONFORMATION OF TETRAHEDRAL INTERMEDIATE FOR ALPHA-CHYMOTRYPSIN-CATALYZED HYDROLYSIS OF PEPTIDE SUBSTRATES
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ZWEIFEL, BO
[J]. FEBS LETTERS, 1975, 59 (01) : 105 - 108
[5] EQUILIBRIUM ASSUMPTION IS VALID FOR THE KINETIC TREATMENT OF MOST TIME-DEPENDENT PROTEIN-MODIFICATION REACTIONS
BROCKLEHURST, K
[J]. BIOCHEMICAL JOURNAL, 1979, 181 (03) : 775 - 778
[6] MECHANISM OF REACTION OF PAPAIN WITH SUBSTRATE-DERIVED DIAZOMETHYL KETONES - IMPLICATIONS FOR DIFFERENCE IN SITE SPECIFICITY OF HALOMETHYL KETONES FOR SERINE PROTEINASES AND CYSTEINE PROTEINASES AND FOR STEREOELECTRONIC REQUIREMENTS IN PAPAIN CATALYTIC MECHANISM
BROCKLEHURST, K
MALTHOUSE, JPG
[J]. BIOCHEMICAL JOURNAL, 1978, 175 (02) : 761 - 764
[7] BROCKLEHURST K, 1979, BIOCHEM J, V183, P369, DOI 10.1042/bj1830369
[8] EVIDENCE THAT BINDING TO THE S-2-SUBSITE OF PAPAIN MAY BE COUPLED WITH CATALYTICALLY RELEVANT STRUCTURAL-CHANGE INVOLVING THE CYSTEINE-25-HISTIDINE-159 DIAD - KINETICS OF THE REACTION OF PAPAIN WITH A 2-PROTONIC-STATE REACTIVITY PROBE CONTAINING A HYDROPHOBIC SIDE-CHAIN
BROCKLEHURST, K
MALTHOUSE, JPG
SHIPTON, M
[J]. BIOCHEMICAL JOURNAL, 1979, 183 (02) : 223 - 231
[9] REACTIONS OF PAPAIN AND OF LOW-MOLECULAR-WEIGHT THIOLS WITH SOME AROMATIC DISULFIDES - 2,2'-DIPYRIDYL DISULFIDE AS A CONVENIENT ACTIVE-SITE TITRANT FOR PAPAIN EVEN IN PRESENCE OF OTHER THIOLS
BROCKLEHURST, K
LITTLE, G
[J]. BIOCHEMICAL JOURNAL, 1973, 133 (01) : 67 - 80
[10] EVIDENCE THAT THE ACTIVE-CENTER OF CHYMOPAPAIN-A IS DIFFERENT FROM THE ACTIVE-CENTERS OF SOME OTHER CYSTEINE PROTEINASES AND THAT THE BRONSTED COEFFICIENT (BETA-NUC.) FOR THE REACTIONS OF THIOLATE ANIONS WITH 2,2'-DIPYRIDYL DISULFIDE MAY BE DECREASED BY REAGENT PROTONATION
BROCKLEHURST, K
BAINES, BS
MUSHIRI, MS
[J]. BIOCHEMICAL JOURNAL, 1980, 189 (01) : 189 - 192

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