GOSSYPOLONE SUPPRESSES PROGESTERONE SYNTHESIS IN BOVINE LUTEAL CELLS

被引：17

作者：

GU, Y

LI, PK

LIN, YC

RIKIHISA, Y

BRUEGGEMEIER, RW

机构：

[1] OHIO STATE UNIV, DEPT VET PHYSIOL & PHARMACOL, REPROD ENDOCRINOL LAB, COLUMBUS, OH 43210 USA

[2] OHIO STATE UNIV, COLL PHARM, DIV MED CHEM & PHARMACOGNOSY, COLUMBUS, OH 43210 USA

[3] OHIO STATE UNIV, COLL VET MED, VET PATHOBIOL LAB, COLUMBUS, OH 43210 USA

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1991年 / 38卷 / 06期

关键词：

D O I：

10.1016/0960-0760(91)90082-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gossypolone, a proposed major metabolite of gossypol, was synthesized and investigated for its effect on progesterone synthesis in cultured bovine luteal cells. Gossypolone inhibited human chorionic gonadotropin(hCG)-stimulated progesterone secretion, reduced substrate-enhanced conversions of 25-hydroxycholesterol to pregnenolone and of pregnenolone to progesterone in a dose-dependent fashion. These findings indicate that gossypolone inhibits not only 3-beta-hydroxysteroid dehydrogenase (3-beta-HSD) activity, as gossypol does, but also side-chain cleavage enzyme complex (cytochrome P450scc) activity. However, the two compounds appear to have a similar potency in inhibiting progesterone secretion. Both gossypolone and gossypol (8.5-mu-M) induced morphological changes in cellular organelles.

引用

页码：709 / 715

页数：7

共 38 条

[1] METABOLIC-FATE OF GOSSYPOL - METABOLISM OF [C-14]GOSSYPOL IN SWINE [J].