IMMUNOAFFINITY-CHROMATOGRAPHY PURIFICATION OF SALBUTAMOL IN LIVER AND HPLC-FLUOROMETRIC DETECTION AT TRACE RESIDUE LEVEL

被引：11

作者：

DEGROODT, JM

DEBUKANSKI, BW

SREBRNIK, S

机构：

[1] Minisére de la Santé Publique et de l'Environnement, Bruxelles, B-1050

来源：

ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG | 1992年 / 195卷 / 06期

关键词：

D O I：

10.1007/BF01204565

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A method combining immunoaffinity-chromatography (IAC) and high-performance liquid chromatography (HPLC) for the analysis of Salbutamol in liver with a low quantification limit of 1 mug/kg has been developed. Salbutamol was extracted with 0.01 mol/L HCl and purified by IAC. The samples were analysed on a liquid chromatograph fitted with a C18 mu-Bondapak column. A fluorometer was used for the detection of salbutamol. Recoveries of 67-80% could be obtained.

引用

页码：566 / 568

页数：3

共 14 条

[1]

DEGROOF J, 1991, Z LEBENSM UNTERS FOR, V193, P126

[2]

FURST P, 1989, DEUT LEBENSM-RUNDSCH, V85, P341

[3] DETERMINATION OF SALBUTAMOL IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION [J].

HUTCHINGS, MJ ;

PAULL, JD ;

MORGAN, DJ .

JOURNAL OF CHROMATOGRAPHY, 1983, 277 (OCT) :423-426

[4] SEPARATION AND SIMULTANEOUS DETERMINATION OF BAMIPINE AND SALBUTAMOL IN DOSAGE FORMS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

KOUNTOURELLIS, JE ;

MARKOPOULOU, C ;

GEORGAKOPOULOS, PP .

JOURNAL OF CHROMATOGRAPHY, 1990, 502 (01) :189-192

[5] DETERMINATION OF BETA-2-RECEPTOR AGONISTS IN BOVINE URINE AND LIVER BY GAS-CHROMATOGRAPHY TANDEM MASS-SPECTROMETRY [J].

LEYSSENS, L ;

DRIESSEN, C ;

JACOBS, A ;

CZECH, J ;

RAUS, J .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1991, 564 (02) :515-527

[6] RESIDUE SCREENING FOR THE BETA-AGONISTS CLENBUTEROL, SALBUTAMOL AND CIMATEROL IN URINE USING ENZYME-IMMUNOASSAY AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

MEYER, HHD ;

RINKE, L ;

DURSCH, I .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1991, 564 (02) :551-556

[7]

MEYER HHD, 1991, J ANIM SCI, V469, P4538

[8] ANALYSIS OF ALBUTEROL IN HUMAN-PLASMA BASED ON IMMUNOAFFINITY CHROMATOGRAPHIC CLEANUP COMBINED WITH HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORIMETRIC DETECTION [J].

ONG, H ;

ADAM, A ;

PERREAULT, S ;

MARLEAU, S ;

BELLEMARE, M ;

DUSOUICH, P ;

BEAULIEU, N .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1989, 497 :213-221

[9] DETERMINATION OF CHLORAMPHENICOL IN SWINE MUSCLE-TISSUE USING A MONOCLONAL ANTIBODY-MEDIATED CLEANUP PROCEDURE [J].

VANDEWATER, C ;

HAAGSMA, N .

JOURNAL OF CHROMATOGRAPHY, 1987, 411 :415-421

[10] ASSAY FOR TRENBOLONE AND ITS METABOLITE 17-ALPHA-TRENBOLONE IN BOVINE URINE BASED ON IMMUNOAFFINITY CHROMATOGRAPHIC CLEANUP AND OFF-LINE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY THIN-LAYER CHROMATOGRAPHY [J].

VANGINKEL, LA ;

VANBLITTERSWIJK, H ;

ZOONTJES, PW ;

VANDENBOSCH, D ;

STEPHANY, RW .

JOURNAL OF CHROMATOGRAPHY, 1988, 445 (02) :385-392

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