PRACTICAL ASPECTS OF CHIRAL SEPARATIONS OF PHARMACEUTICALS BY CAPILLARY ELECTROPHORESIS .1. SEPARATION OPTIMIZATION

被引:47
作者
GUTTMAN, A
COOKE, N
机构
[1] Beckman Instruments, Inc., Fullerton, CA 92634
关键词
D O I
10.1016/0021-9673(94)80063-4
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Capillary electrophoresis employing chiral selectors has been shown to be a useful analytical method to separate enantiomers. In our model system hydroxypropyl-beta-cyclodextrin was used as chiral selector for the separation of racemic propranolol. Results are presented regarding the effect of different operational variables such as buffer pH, concentration of chiral selector, applied electric field and temperature on the chiral separation. Based on the experimental data, the operational variables were optimized to attain maximum resolving power with minimal analysis time.
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页码:157 / 162
页数:6
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