REGULATION OF CARDIAC INSULIN-RECEPTOR FUNCTION BY GUANOSINE NUCLEOTIDES

被引：13

作者：

RUSS, M ^{[1
]}

REINAUER, H ^{[1
]}

ECKEL, J ^{[1
]}

机构：

[1] DIABET FORSCHUNGSINST,MOLEC CARDIOL LAB,HENNEKAMP 65,W-4000 DUSSELDORF 1,GERMANY

来源：

FEBS LETTERS | 1992年 / 314卷 / 01期

关键词：

ISOLATED CARDIAC MYOCYTE; INSULIN RECEPTOR; GUANOSINE NUCLEOTIDE BINDING PROTEIN; TYROSINE KINASE;

D O I：

10.1016/0014-5793(92)81464-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study examined the effect of [S-35]GTP on the function of insulin receptors partially purified from adult rat cardiomyocytes by WGA chromatography. [S-35]GTP increased receptor autophosphorylation about two times and fully mimicked the stimulatory action of insulin on poly(Glu:Tyr) phosphorylation with no additional effect of the hormone. The effect of [S-35]GTP was specific, dose-dependent, and due to an increase in the V(max) of the kinase. In the presence of ATP or AMP-PNP. insulin significantly enhanced the binding of [S-35]GTP to the partially purified insulin receptor. The findings suggest coupling of the insulin receptor to a G-protein which may be involved in the regulation of tyrosine kinase activity.

引用

页码：72 / 76

页数：5

共 28 条

[11] INSULIN ACTIVATES GTP BINDING TO A 40-KDA PROTEIN IN FAT-CELLS
KELLERER, M
OBERMAIERKUSSER, B
PROFROCK, A
SCHLEICHER, E
SEFFER, E
MUSHACK, J
ERMEL, B
HARING, HU
[J]. BIOCHEMICAL JOURNAL, 1991, 276 : 103 - 108
[12] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
LAEMMLI, UK
[J]. NATURE, 1970, 227 (5259) : 680 - +
[13] LEFKOWITZ RJ, 1988, J BIOL CHEM, V263, P4993
[14] LUTTRELL L, 1990, J BIOL CHEM, V265, P16873
[15] LUTTRELL LM, 1988, J BIOL CHEM, V263, P6134
[16] MADDUX BA, 1991, J BIOL CHEM, V266, P6731
[17] GUANOSINE NUCLEOTIDES REGULATE HORMONE BINDING OF INSULIN-RECEPTORS
MORTENSEN, ER
DRACHMAN, J
GUIDOTTI, G
[J]. BIOCHEMICAL JOURNAL, 1992, 281 : 735 - 743
[18] PERTUSSIS TOXIN INHIBITS AUTOPHOSPHORYLATION AND ACTIVATION OF THE INSULIN-RECEPTOR KINASE
MULLERWIELAND, D
WHITE, MF
BEHNKE, B
GEBHARDT, A
NEUMANN, S
KRONE, W
KAHN, CR
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 181 (03) : 1479 - 1485
[19] GUANINE-NUCLEOTIDES INDUCE TYROSINE PHOSPHORYLATION AND ACTIVATION OF THE RESPIRATORY BURST IN NEUTROPHILS
NASMITH, PE
MILLS, GB
GRINSTEIN, S
[J]. BIOCHEMICAL JOURNAL, 1989, 257 (03) : 893 - 897
[20] NISHIMOTO I, 1989, J BIOL CHEM, V264, P14029

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