ENZYMATIC-SYNTHESIS OF THE DELICIOUS PEPTIDE-FRAGMENTS IN EUTECTIC MIXTURES

The feasibility of flavor peptide production has been investigated using proteases in liquid/semiliquid eutectic mixtures comprised of the substrates together with small amounts of water and alcohols (19-24% w/w). The three functional fragments of the so-called ''Delicious Octapeptide'', L-Lys-Gly, L-Asp-Glu-Glu, and L-Ser-Leu-Ala, were obtained in good yields without any requirement for the regioselective side-chain protection of multifunctional amino acids. The peptides were synthesized on a preparative scale in overall yields of 29-77% and were fully characterized.