WATER ACTIVITY DOES NOT INFLUENCE THE ENANTIOSELECTIVITY OF LIPASE PS AND LIPOPROTEIN-LIPASE IN ORGANIC-SOLVENTS

被引：55

作者：

BOVARA, R ^{[1
]}

CARREA, G ^{[1
]}

OTTOLINA, G ^{[1
]}

RIVA, S ^{[1
]}

机构：

[1] CNR,IST CHIM ORMONI,VIA MARIO BIANCO 9,I-20131 MILAN,ITALY

来源：

BIOTECHNOLOGY LETTERS | 1993年 / 15卷 / 02期

关键词：

D O I：

10.1007/BF00133018

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The activity and enantioselectivity of Lipase PS from Pseudomonas cepacia and lipoprotein lipase from Pseudomonas sp. were investigated in organic solvents preequilibrated to water activities ranging from <0.1 to 0.53, using as a model reaction the transesterification between (+/-)-sulcatol and vinyl acetate. Variations of water activity markedly influenced the transesterification rate but did not modify the enantioselectivity of the two enzymes.

引用

页码：169 / 174

页数：6

共 22 条

[1] ON THE IMPORTANCE OF THE SUPPORT MATERIAL FOR ENZYMATIC-SYNTHESIS IN ORGANIC MEDIA - SUPPORT EFFECTS AT CONTROLLED WATER ACTIVITY
ADLERCREUTZ, P
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 199 (03): : 609 - 614
[2] RESOLUTION OF (+/-)-TRANS-SOBREROL BY LIPASE PS-CATALYZED TRANSESTERIFICATION AND EFFECTS OF ORGANIC-SOLVENTS ON ENANTIOSELECTIVITY
BOVARA, R
CARREA, G
FERRARA, L
RIVA, S
[J]. TETRAHEDRON-ASYMMETRY, 1991, 2 (09) : 931 - 938
[3] PREPARATIVE PRODUCTION OF OPTICALLY-ACTIVE ESTERS AND ALCOHOLS USING ESTERASE-CATALYZED STEREOSPECIFIC TRANS-ESTERIFICATION IN ORGANIC MEDIA
CAMBOU, B
KLIBANOV, AM
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1984, 106 (09) : 2687 - 2692
[4] GENERAL-ASPECTS AND OPTIMIZATION OF ENANTIOSELECTIVE BIOCATALYSIS IN ORGANIC-SOLVENTS - THE USE OF LIPASES
CHEN, CS
SIH, CJ
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1989, 28 (06): : 695 - 707
[5] CHEMOENZYMATIC SYNTHESIS OF CHIRAL ISOXAZOLE DERIVATIVES
DEAMICI, M
DEMICHELI, C
CARREA, G
SPEZIA, S
[J]. JOURNAL OF ORGANIC CHEMISTRY, 1989, 54 (11) : 2646 - 2650
[6] HOW CAN THE SOLVENT AFFECT ENZYME ENANTIOSELECTIVITY
FITZPATRICK, PA
KLIBANOV, AM
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (08) : 3166 - 3171
[7] LIPASE-CATALYZED ESTER EXCHANGE-REACTIONS IN ORGANIC MEDIA WITH CONTROLLED HUMIDITY
GODERIS, HL
AMPE, G
FEYTEN, MP
FOUWE, BL
GUFFENS, WM
VANCAUWENBERGH, SM
TOBBACK, PP
[J]. BIOTECHNOLOGY AND BIOENGINEERING, 1987, 30 (02) : 258 - 266
[8] HUMIDITY FIXED-POINTS OF BINARY SATURATED AQUEOUS-SOLUTIONS
GREENSPAN, L
[J]. JOURNAL OF RESEARCH OF THE NATIONAL BUREAU OF STANDARDS SECTION A-PHYSICS AND CHEMISTRY, 1977, 81 (01): : 89 - 96
[9] TEMPERATURE AS AN ENANTIOSELECTIVE PARAMETER IN ENZYMATIC RESOLUTIONS OF RACEMIC MIXTURES
HOLMBERG, E
HULT, K
[J]. BIOTECHNOLOGY LETTERS, 1991, 13 (05) : 323 - 326
[10] Holmberg E., 1990, BIOCATALYSIS, V3, P243

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