DETECTION OF ESCHERICHIA-COLI O157-H7 IN MEAT BY AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY, EHEC-TEK

被引：67

作者：

JOHNSON, RP

DURHAM, RJ

JOHNSON, ST

MACDONALD, LA

JEFFREY, SR

BUTMAN, BT

机构：

[1] ORGANON TEKNIKA CORP,BIOTECHNOL RES INST,ROCKVILLE,MD 20850

[2] ORGANON TEKNIKA CORP,DURHAM,NC 27712

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 01期

关键词：

D O I：

10.1128/AEM.61.1.386-388.1995

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Investigation of the specificity of an enzyme-linked immunosorbent assay (ELISA) for detection of Escherichia coli O157:H7 in raw meats (EHEC-Tek; Organon Teknika Corp.) revealed that the target antigens of the detection reagent; monoclonal antibody 4E8C12, were present in numerous serotypes of E. coli and that their ELISA reactivity was influenced by bile salts, acriflavine, and heat. The specificity of the ELISA was improved by a modified test protocol incorporating immunocapture.

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收藏

页码：386 / 388

页数：3

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