IDENTIFICATION AND CHARACTERIZATION OF STYLAR GLYCOPROTEINS ASSOCIATED WITH SELF-INCOMPATIBILITY GENES OF JAPANESE PEAR, PYRUS-SEROTINA REHD

被引:100
作者
SASSA, H [1 ]
HIRANO, H [1 ]
IKEHASHI, H [1 ]
机构
[1] NATL INST AGROBIOL RESOURCES,DEPT MOLEC BIOL,TSUKUBA,IBARAKI 305,JAPAN
来源
MOLECULAR & GENERAL GENETICS | 1993年 / 241卷 / 1-2期
关键词
GAMETOPHYTIC SELF-INCOMPATIBILITY; JAPANESE PEAR; PYRUS-SEROTINA REHD; S-GLYCOPROTEIN; N-TERMINAL AMINO ACID SEQUENCE;
D O I
10.1007/BF00280196
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Japanese pear (Pyrus serotina Rehd.) exhibits gametophytic self-incompatibility. Following our previous findings that basic ribonucleases in the styles of Japanese pear are associated with self-incompatibility genes (S-RNases), stylar proteins with high pl values were analyzed by two-dimensional gel electrophoresis further to characterize S-RNases. A group of basic proteins of about 30 kDa associated with self-incompatibility genes were identified. These proteins contained sugar chains which reacted with concanavalin A and wheat germ agglutinin, and thus were designated as S-glycoproteins of Japanese pear. The fact that the S-glycoprotein was expressed at a much lower level in a self-compatible mutant than in the original variety suggested a role of S-glycoproteins in mediating self-incompatibility of Japanese pear. Immunoblot analysis indicated that S-glycoproteins are identical to previously identified S-RNases. The S-glycoproteins were predominantly expressed in the style, in the ovary in trace amounts, and not in leaf, pollen or germinated pollen. The N-terminal amino acid sequences of the S-glycoproteins showed homology not only with each other but also with those of the S-allele-associated proteins from plants of the family Solanaceae at levels of about 30-50%.
引用
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页码:17 / 25
页数:9
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