CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF NONGLYCOSYLATED TISSUE INHIBITOR OF METALLOPROTEINASES-1, N30QN78Q TIMP-1

被引：10

作者：

TOLLEY, SP

DAVIES, GJ

OSHEA, M

COCKETT, MI

DOCHERTY, AJP

MURPHY, G

机构：

[1] STRANGEWAYS RES LAB,DEPT CELL & MOLEC BIOL,CAMBRIDGE CB1 4RN,ENGLAND

[2] CELLTECH LTD,SLOUGH SL1 4EN,ENGLAND

来源：

PROTEINS-STRUCTURE FUNCTION AND GENETICS | 1993年 / 17卷 / 04期

基金：

英国惠康基金;

关键词：

METALLOPROTEINASE; INHIBITOR; X-RAY DIFFRACTION; TUMOR INVASION;

D O I：

10.1002/prot.340170410

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A nonglycosylated (N30QN78Q) form of the human tissue inhibitor of metalloproteinases, TIMP-1, has been prepared and crystallized in a form suitable for X-ray diffraction analysis. Small single crystals have been grown using sodium tartrate as a precipitant. The crystals are in space group P2(1), with cell dimensions a = 35.28, b = 53.95, c = 48.56, and beta = 96.0-degrees. There is a single molecule of TIMP-1 in the asymmetric unit. The crystals diffract to at least 2.3 angstrom resolution. Complete data have been collected to 2.9 angstrom and a search for heavy-metal derivatives is in progress. (C) 1993 Wiley-Liss, Inc.

引用

页码：435 / 437

页数：3

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