SOME FEATURES OF THE STREPTOCOCCUS-FAECALIS NA+-ATPASE RESEMBLE THOSE OF THE VACUOLAR-TYPE ATPASES

被引：41

作者：

KAKINUMA, Y

IGARASHI, K

机构：

[1] Faculty of Pharmaceutical Sciences, Chiba University, Chiba, 260

来源：

FEBS LETTERS | 1990年 / 271卷 / 1-2期

关键词：

Induction; Na[!sup]+[!/sup]-ATPase; Streptococcus faecalis; Vacuolar ATPase;

D O I：

10.1016/0014-5793(90)80381-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the ethylenediaminetetraacetic acid (EDTA) extract prepared from the membranes of Streptococcus faecalis, we found the 330-kDa protein that was coordinately increased with the induction of Na+-ATPase. It was missed in the EDTA extract of Nakl, a mutant defective in the Na+-ATPase, but restored in that of its revertant, NaklR. The 330-kDa protein showed the ATP hydrolytic activity by active staining, and mainly consisted of the polypeptides of 73 kDa, 52 kDa and possibly 38 kDa. In addition, the Na+-stimulated ATPase of the membranes was sensitive to both nitrate and N-ethylmaleimide, inhibitors for the vacuolar H+-ATPase. Thus, the Na+-ATPase of this organism has a structure similar to vacuolar H+-ATPase. © 1990.

引用

页码：97 / 101

页数：5

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