RELEASE OF THE COMPONENT OF STREPTOCOCCUS-FAECALIS NA+-ATPASE FROM THE MEMBRANES

被引：15

作者：

KAKINUMA, Y

IGARASHI, K

机构：

[1] Faculty of Pharmaceutical Sciences, Chiba University, Chiba, 260

来源：

FEBS LETTERS | 1990年 / 271卷 / 1-2期

关键词：

Induction; Na[!sup]+[!/sup]-ATPase; Reconstitution; Streptococcus faecalis;

D O I：

10.1016/0014-5793(90)80382-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Na+-stimulated ATPase activity of Streptococcus faecalis was lost by washing the membranes with ethylenediaminetetraacetic acid (EDTA). ATPase activities of both the EDTA extract and the stripped membranes did not show any stimulation by Na+ ions. However, the Na+-stimulated ATPase was readily reconstituted by an incubation of these fractions combined. It was only reconstituted from the fractions prepared under the condition that the Na+-ATPase is amplified, and not from those boiled or digested by trypsin. Thus, the component of Na+-ATPase of this organism is capable of being released from the membranes. © 1990.

引用

页码：102 / 105

页数：4

共 16 条

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[2]

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[4]

DIVROV PA, 1988, FEBS LETT, V233, P355