FRACTIONATION OF CELLULASES FROM THE RUMINAL FUNGUS NEOCALLIMASTIX-FRONTALIS EB188

被引：31

作者：

LI, XL ^{[1
]}

CALZA, RE ^{[1
]}

机构：

[1] WASHINGTON STATE UNIV, DEPT ANIM SCI, PULLMAN, WA 99164 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1991年 / 57卷 / 11期

关键词：

D O I：

10.1128/AEM.57.11.3331-3336.1991

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cellulases from the ruminal fungus Neocallimastix frontalis EB188 were separated by using hydroxylapatite column chromatography. Seven carboxymethylcellulases, six avicelases, and four beta-glucosidases accounted for the majority of the activities. The separation of enzymes was confirmed by using polyacrylamide gel electrophoresis. Electrophoretic migration, analysis of hydrolysis products, and substrate specificity measurements suggested that several different cellulases were secreted in N. frontalis EB188. The possible relationship of cellulase diversity to protein glycosylation is discussed.

引用

页码：3331 / 3336

页数：6

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