SYNTHESIS OF HIGH ERUCIC-ACID RAPESEED (BRASSICA-NAPUS L) SOMATIC HYBRIDS WITH IMPROVED AGRONOMIC CHARACTERS

被引：14

作者：

HEATH, DW ^{[1
]}

EARLE, ED ^{[1
]}

机构：

[1] CORNELL UNIV, DEPT PLANT BREEDING & BIOMETRY, ITHACA, NY 14853 USA

来源：

THEORETICAL AND APPLIED GENETICS | 1995年 / 91卷 / 6-7期

关键词：

BRASSICA NAPUS; SOMATIC HYBRIDS; PROTOPLAST FUSION; ERUCIC ACID;

D O I：

10.1007/BF00223931

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Novel Brassica napus somatic hybrids have been created through protoplast fusion of B. oleracea var. botrytis and B. rapa var, oleifera genotypes selected for high erucic acid (22:1) content in the seed oil. Fifty amphidiploids (aacc) and one putative hexaploid (aacccc) hybrid were recovered in one fusion experiment. Conversely, only one amphidiploid and numerous regenerates with higher DNA contents were produced in a similar fusion using a different B. rapa partner. Hybridity was confirmed by morphology, isozyme expression, flow cytometry, and DNA hybridization. Analysis of organellar DNA revealed a distinct bias toward the inheritance of chloroplasts from the B. rapa (aa) genome. All amphidiploids set self-pollinated seed. A erucic acid content as high as 57.4% was found in the seed oil of one regenerated plant. Fatty acid composition was stable in the R(1) generation and was coupled with increased female fertility. Other novel agronomic characters in the hybrids recovered include large seed size, lodging resistance, and non-shattering seed pods.

引用

页码：1129 / 1136

页数：8

共 34 条

[21] INTERGENERIC CYTOPLASMIC HYBRIDIZATION IN CRUCIFERAE BY PROTOPLAST FUSION [J].

PELLETIER, G ;

PRIMARD, C ;

VEDEL, F ;

CHETRIT, P ;

REMY, R ;

ROUSSELLE ;

RENARD, M .

MOLECULAR & GENERAL GENETICS, 1983, 191 (02) :244-250

[22]

PRYDE EH, 1989, OIL CROPS WORLD, P87

[23] INTERSPECIFIC HYBRIDS BETWEEN BRASSICA-NAPUS L AND BRASSICA-OLERACEA L DEVELOPED BY EMBRYO CULTURE [J].

QUAZI, MH .

THEORETICAL AND APPLIED GENETICS, 1988, 75 (02) :309-318

[24] ANALYSIS OF ORGANELLE GENOMES IN A SOMATIC HYBRID DERIVED FROM CYTOPLASMIC MALE-STERILE BRASSICA-OLERACEA AND ATRAZINE-RESISTANT BRASSICA-CAMPESTRIS [J].

ROBERTSON, D ;

PALMER, JD ;

EARLE, ED ;

MUTSCHLER, MA .

THEORETICAL AND APPLIED GENETICS, 1987, 74 (03) :303-309

[25] PLANT-REGENERATION FROM LEAF PROTOPLASTS OF BRASSICA-OLERACEA VAR ITALICA CV GREEN COMET BROCCOLI [J].

ROBERTSON, D ;

EARLE, ED .

PLANT CELL REPORTS, 1986, 5 (01) :61-64

[26]

SCHENCK HR, 1982, Z PFLANZENZUCHT, V89, P278

[27]

SHELDRAKE R, 1973, IB43 CORN U NY STAT

[28] CHLOROPLAST TRANSFER IN NICOTIANA BASED ON METABOLIC COMPLEMENTATION BETWEEN IRRADIATED AND IODOACETATE TREATED PROTOPLASTS [J].

SIDOROV, VA ;

MENCZEL, L ;

NAGY, F ;

MALIGA, P .

PLANTA, 1981, 152 (04) :341-345

[29] FERTILITY AND CHROMOSOME STABILITY IN BRASSICA-NAPUS RESYNTHESIZED BY PROTOPLAST FUSION [J].

SUNDBERG, E ;

LANDGREN, M ;

GLIMELIUS, K .

THEORETICAL AND APPLIED GENETICS, 1987, 75 (01) :96-104

[30] EFFECTS OF PARENTAL PLOIDY LEVEL AND GENETIC-DIVERGENCE ON CHROMOSOME ELIMINATION AND CHLOROPLAST SEGREGATION IN SOMATIC HYBRIDS WITHIN BRASSICACEAE [J].

SUNDBERG, E ;

GLIMELIUS, K .

THEORETICAL AND APPLIED GENETICS, 1991, 83 (01) :81-88

← 1 2 3 4 →