MODIFICATION OF TRANSPLASMA MEMBRANE OXIDOREDUCTION BY SV40 TRANSFORMATION OF 3T3 CELLS

被引：22

作者：

LOW, H ^{[1
]}

CRANE, FL ^{[1
]}

GREBING, C ^{[1
]}

ISAKSSON, M ^{[1
]}

LINDGREN, A ^{[1
]}

SUN, IL ^{[1
]}

机构：

[1] PURDUE UNIV,DEPT BIOL SCI,W LAFAYETTE,IN 47907

来源：

JOURNAL OF BIOENERGETICS AND BIOMEMBRANES | 1991年 / 23卷 / 06期

关键词：

TRANSFERRIN RECEPTOR; DIFERRIC TRANSFERRIN; 3T3; CELLS; TRANSFORMATION; IRON REDUCTION; PLASMA MEMBRANE;

D O I：

10.1007/BF00786008

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Transformation of 3T3 cells by SV40 virus changes the properties of the transplasma membrane electron transport activity which can be assayed by reduction of external ferric salts. After 42 h of culture and before the growth rate is maximum, the transformed cells have a much slower rate of ferric reduction. The change in activity is expressed both by change in K(m) and V(max) for ferricyanide reduction. The change in activity is not based on surface charge effect or on tight coupling to proton release or on intracellular NADH concentration. With transformation by SV40 virus infection the expression of transferrin receptors increases, which correlates with greater diferric transferrin stimulation of the rate of ferric ammonium citrate reduction in transformed SV40-3T3 cells than in 3T3 cells.

引用

页码：903 / 917

页数：15

共 53 条

[11]

EKBOM P, 1983, INT J CANCER, V31, P111

[12] FERRICYANIDE CAN REPLACE PYRUVATE TO STIMULATE GROWTH AND ATTACHMENT OF SERUM RESTRICTED HUMAN-MELANOMA CELLS [J].