INTERGENERIC TRANSFER OF CYTOPLASMIC MALE-STERILITY BETWEEN RAPHANUS-SATIVUS (CMS LINE) AND BRASSICA-NAPUS THROUGH CYTOPLAST-PROTOPLAST FUSION

被引:65
作者
SAKAI, T
IMAMURA, J
机构
[1] Plantech Research Institute, Yokohama, 227, 1000 Kamoshida, Midori-ku
关键词
B; napus; Cybrid cms; Cytoplast; Fusion; transfer;
D O I
10.1007/BF00210083
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Cytoplasts isolated from hypocotyl protoplasts of Raphanus sativus cv Kosena (cms line) by ultracentrifugation through Percoll/mannitol discontinuous gradient were fused with iodoacetamide(IOA)-treated protoplasts of Brassica napus cv Westar. Seventeen randomly selected regenerated plants were characterized for morphology and chromosome numbers. All of the regenerated plants had morphology identical to B. napus and 10 of them possessed the diploid chromosome number of B. napus. The remaining plants had chimeric or aneuploid chromosome numbers. The mitochondrial genomes in the 10 fusion products possessing the diploid chromosome numbers of B. napus were examined by Southern hybridization analysis. Four of the 10 plants contained mitochondrial DNA showing novel hybridization patterns. Of these 4 plants, 1 was male sterile, and 3 were male fertile. The remaining plants showed mitochondrial DNA patterns identical to B. napus and were male fertile. © 1990 Springer-Verlag.
引用
收藏
页码:421 / 427
页数:7
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