IMMOBILIZED LIPOSOME CHROMATOGRAPHY FOR ANALYSIS OF INTERACTIONS BETWEEN LIPID BILAYERS AND PEPTIDES

被引:32
作者
ZHANG, YX
AIMOTO, S
LU, L
YANG, Q
LUNDAHL, P
机构
[1] UNIV UPPSALA,CTR BIOMED,DEPT BIOCHEM,S-75123 UPPSALA,SWEDEN
[2] OSAKA UNIV,INST PROT RES,SUITA,OSAKA 565,JAPAN
关键词
D O I
10.1006/abio.1995.1415
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Liposomes were sterically immobilized in gel beads to be used for isocratic chromatographic analysis of interactions between lipid bilayers, amino acids, and water-soluble peptides. Tryptophan was more retarded on the immobilized liposomes than were other amino acids, and peptides with C-terminal cysteine were much more retarded than were peptides with serine or aminobutyric acid instead of the cysteine, Peptide sequences could affect the peptide-liposome interactions. For peptides corresponding to polypeptide segments of a membrane protein, the human red cell glucose transporter (Glut1), the retention volumes increased with decreasing water-to-oil transfer free energy of the peptides and were related to the transfer free energy distribution within the peptides as shown by hydropathy plots and to the presence of cysteine, Among these peptides, the partially hydrophobic peptides (125)GRFIIGVCG(134) and (201)CIVLPFCPES(210) and the hydrophilic peptide (421)CFQYVEQLC(429) were most strongly retarded on immobilized phosphatidylcholine liposomes. The results indicated that the peptide-liposome interactions were mainly of hydrophobic nature and that the structure of the interfacial headgroup region of the lipid bilayers was important. (C) 1995 Academic Press, Inc.
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页码:291 / 298
页数:8
相关论文
共 44 条
[32]  
SCHWYZER R, 1992, BRAZ J MED BIOL RES, V25, P1077
[33]  
SWAISGOOD HE, 1987, ANAL AFFINITY CHROMA, P65
[34]  
THANNHAUSER TW, 1987, METHOD ENZYMOL, V143, P115
[35]   ENTRAPMENT OF LIPID VESICLES AND MEMBRANE PROTEIN-LIPID VESICLES IN GEL BEAD PORES [J].
WALLSTEN, M ;
YANG, Q ;
LUNDAHL, P .
BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 982 (01) :47-52
[36]   THE DIFFERENTIAL ROLE OF CYS-421 AND CYS-429 OF THE GLUT1 GLUCOSE TRANSPORTER IN TRANSPORT INHIBITION BY P-CHLOROMERCURIBENZENESULFONIC ACID (PCMBS) OR CYTOCHALASIN-B (CB) [J].
WELLNER, M ;
MONDEN, I ;
KELLER, K .
FEBS LETTERS, 1992, 309 (03) :293-296
[37]   PEPTIDES IN LIPID BILAYERS - STRUCTURAL AND THERMODYNAMIC BASIS FOR PARTITIONING AND FOLDING [J].
WHITE, SH ;
WIMLEY, WC .
CURRENT OPINION IN STRUCTURAL BIOLOGY, 1994, 4 (01) :79-86
[38]   STRUCTURE OF A FLUID DIOLEOYLPHOSPHATIDYLCHOLINE BILAYER DETERMINED BY JOINT REFINEMENT OF X-RAY AND NEUTRON-DIFFRACTION DATA .3. COMPLETE STRUCTURE [J].
WIENER, MC ;
WHITE, SH .
BIOPHYSICAL JOURNAL, 1992, 61 (02) :434-447
[39]  
WINZOR DJ, 1985, AFFINITY CHROMATOGR, P149
[40]   LIPID-VESICLE-SURFACE CHROMATOGRAPHY [J].
YANG, Q ;
WALLSTEN, M ;
LUNDAHL, P .
JOURNAL OF CHROMATOGRAPHY, 1990, 506 :379-389