ISOLATION OF TYPE-I AND TYPE-II GABA-A-BENZODIAZEPINE RECEPTORS BY IMMUNOAFFINITY CHROMATOGRAPHY

被引：49

作者：

ZEZULA, J ^{[1
]}

SIEGHART, W ^{[1
]}

机构：

[1] UNIV VIENNA,PSYCHIAT KLIN,DEPT BIOCHEM PSYCHIAT,WAHRINGER GURTEL 18-20,A-1090 VIENNA,AUSTRIA

来源：

FEBS LETTERS | 1991年 / 284卷 / 01期

关键词：

GABA-A-BENZODIAZEPINE RECEPTOR; IMMUNOAFFINITY CHROMATOGRAPHY; TYPE-I AND TYPE-II GABA-A RECEPTOR; FLUNITRAZEPAM BINDING; AUTORADIOGRAPHY;

D O I：

10.1016/0014-5793(91)80750-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Anti-peptide alpha-1 (1-9) and anti-peptide alpha-3 (459-467) antibodies coupled to Affigel-10 were used for the isolation of GABA(A) receptors containing the alpha-1- or alpha-3-subunit, respectively. Both types of GABA(A) receptors exhibited a high affinity for [H-3]flunitrazepam, and binding of [H-3]flunitrazepam was stimulated in the presence of GABA. GABA(A) receptors eluted from the anti-peptide alpha-1 (1-9) immunoaffinity column exhibited a high affinity and those from the anti-peptide alpha-3 (459-467) columns a low affinity for the type I benzodiazepine receptor-selective ligand Cl 218872, indicating the enrichment of type I and type II GABA(A)-benzodiazepine receptors, respectively.

引用

页码：15 / 18

页数：4

共 11 条

[1]

DUGGAN MJ, 1990, J BIOL CHEM, V265, P3831

[2] SUBUNIT SELECTIVITY AND EPITOPE CHARACTERIZATION OF MABS DIRECTED AGAINST THE GABA-A BENZODIAZEPINE RECEPTOR [J].

EWERT, M ;

SHIVERS, BD ;

LUDDENS, H ;

MOHLER, H ;

SEEBURG, PH .

JOURNAL OF CELL BIOLOGY, 1990, 110 (06) :2043-2048

[3] IDENTIFICATION OF ALPHA-2-SUBUNIT AND ALPHA-3-SUBUNIT OF THE GABA-A-BENZODIAZEPINE RECEPTOR COMPLEX PURIFIED FROM THE BRAINS OF YOUNG-RATS [J].

FUCHS, K ;

ADAMIKER, D ;

SIEGHART, W .

FEBS LETTERS, 1990, 261 (01) :52-54

[4] STRUCTURAL AND FUNCTIONAL BASIS FOR GABAA RECEPTOR HETEROGENEITY [J].

LEVITAN, ES ;

SCHOFIELD, PR ;

BURT, DR ;

RHEE, LM ;

WISDEN, W ;

KOHLER, M ;

FUJITA, N ;

RODRIGUEZ, HF ;

STEPHENSON, A ;

DARLISON, MG ;

BARNARD, EA ;

SEEBURG, PH .

NATURE, 1988, 335 (6185) :76-79

[5]

MOHLER H, 1990, OCT LECT S TRANSM AM

[6]

OLSEN RW, 1986, RECEPTOR BIOCH METHO, V5

[7] TYPE-I AND TYPE-II GABAA-BENZODIAZEPINE RECEPTORS PRODUCED IN TRANSFECTED CELLS [J].

PRITCHETT, DB ;

LUDDENS, H ;

SEEBURG, PH .

SCIENCE, 1989, 245 (4924) :1389-1392

[8] IMPORTANCE OF A NOVEL GABAA RECEPTOR SUBUNIT FOR BENZODIAZEPINE PHARMACOLOGY [J].

PRITCHETT, DB ;

SONTHEIMER, H ;

SHIVERS, BD ;

YMER, S ;

KETTENMANN, H ;

SCHOFIELD, PR ;

SEEBURG, PH .

NATURE, 1989, 338 (6216) :582-585

[9] UNDERSTANDING THE GABAA RECEPTOR - A CHEMICALLY GATED ION CHANNEL [J].

STEPHENSON, FA .

BIOCHEMICAL JOURNAL, 1988, 249 (01) :21-32

[10] IDENTIFICATION OF THE ALPHA-3-SUBUNIT IN THE GABAA RECEPTOR PURIFIED FROM BOVINE BRAIN [J].

STEPHENSON, FA ;

DUGGAN, MJ ;

CASALOTTI, SO .

FEBS LETTERS, 1989, 243 (02) :358-362

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