INDUCTION OF FERREDOXIN-NADP+ OXIDOREDUCTASE AND FERREDOXIN SYNTHESIS IN PEA ROOT PLASTIDS DURING NITRATE ASSIMILATION

被引:46
作者
BOWSHER, CG [1 ]
HUCKLESBY, DP [1 ]
EMES, MJ [1 ]
机构
[1] UNIV MANCHESTER,DEPT CELL & STRUCT BIOL,PLANT METAB RES UNIT,WILLIAMSON BLDG,MANCHESTER M13 9PL,LANCS,ENGLAND
关键词
D O I
10.1111/j.1365-313X.1993.tb00166.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A 14.5 kDa protein with antigenic components in common with pea leaf ferredoxin was detected on transblots of the soluble proteins of pea root plastids. The amount of this protein was found to increase during the induction of nitrate assimilation in pea roots, reaching a maximal level at 8-12 h. Concurrent with this, a fourfold increase in NADPH-dependent ferredoxin-NADP+ oxidoreductase (FNR) activity was observed corresponding to an increase in the amount of this protein detected immunologically on transblots using a leaf FNR antibody. These changes were not observed in plastids from roots of plants grown on ammonia or depleted of nitrogen. It is suggested that in addition to the already well reported induction by nitrate of nitrate reductase and nitrite reductase, there is a co-induction of a plastid located ferredoxin and FNR. Both these proteins are necessary for the transfer of reductant generated by the oxidative pentose phosphate pathway to nitrite reductase.
引用
收藏
页码:463 / 467
页数:5
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