DIFFERENTIAL CONTROL OF FREE CALCIUM AND FREE ZINC LEVELS IN ISOLATED BOVINE LIVER NUCLEI

被引：39

作者：

HECHTENBERG, S ^{[1
]}

BEYERSMANN, D ^{[1
]}

机构：

[1] UNIV BREMEN,FACHBEREICH BIOL CHEM,LEOBENER STR,W-2800 BREMEN 33,GERMANY

来源：

BIOCHEMICAL JOURNAL | 1993年 / 289卷

关键词：

D O I：

10.1042/bj2890757

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Modulation of intracellular signal transduction by Ca2+ and possibly Zn2+ is based on an effective homoeostatic control of the corresponding free ion concentrations. We used the fluorescent indicator fura-2 to monitor concentrations of free Ca2+ and free Zn2+ in nuclei isolated from bovine liver. The nuclei showed an ATP-stimulated accumulation of intranuclear free Ca2+, which was inhibited in the presence of the Ca2+-pump inhibitor thapsigargin. Furthermore, uptake and intranuclear levels of free Zn2+ were measured after incubation with different extranuclear Zn2+ concentrations. There was no stimulating effect of ATP on Zn2+ uptake. Our data suggest that the levels of intranuclear free Ca2+ and free Zn2+ are controlled differentially. A distinct regulation of free ion levels in the nucleus may contribute to the specific control of nuclear events associated with gene transcription and cell differentiation.

引用

页码：757 / 760

页数：4

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