EFFECTS OF L-SERINE ON NEURONS IN-VITRO

被引：52

作者：

SAVOCA, R ^{[1
]}

ZIEGLER, U ^{[1
]}

SONDEREGGER, P ^{[1
]}

机构：

[1] UNIV ZURICH,INST BIOCHEM,CH-8057 ZURICH,SWITZERLAND

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1995年 / 61卷 / 1-2期

基金：

美国国家科学基金会;

关键词：

SERINE; DORSAL ROOT GANGLION NEURON; RETINA; CELL CULTURE; NEURITE GROWTH; AMINO ACID; (CHICKEN);

D O I：

10.1016/0165-0270(95)00038-V

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have examined the influence of amino acids on chicken embryonic dorsal root ganglion (DRG) neurons in vitro and have found that serine has a marked impact on the morphology of the developing neurites. In cultures supplemented with L-serine (but not D-serine) at micromolar concentrations (10-200 mu M), the length of the neurites was increased by up to 100% and they were seen to develop a more complex branching pattern. These effects of L-serine were found to be concentration-dependent and stereospecific and were observed on several different substrata such as laminin, Ng-CAM and axonin-1. Similar observations were also made in the case of embryonic retinal explants, while the addition of non-essential amino acids other than L-serine to DRG neurons was found to have no effect. We conclude that, although belonging to the group of non-essential amino acids and not a recognized neurotransmitter, L-serine is an important factor for the morphological differentiation of neurons in vitro.

引用

页码：159 / 167

页数：9

共 30 条

[1] PURIFICATION OF THE CHICK EYE CILIARY NEURONOTROPHIC FACTOR [J].