STRUCTURE AND TRANSCRIPTION OF A P-ATPASE GENE FROM TRYPANOSOMA-BRUCEI

被引：25

作者：

REVELARD, P ^{[1
]}

PAYS, E ^{[1
]}

机构：

[1] UNIV BRUSSELS,DEPT MOLEC BIOL,67 RUE CHEVAUX,B-1640 RHODE ST GENESE,BELGIUM

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1991年 / 46卷 / 02期

关键词：

TRYPANOSOME; ATPASE GENE; GENE EXPRESSION;

D O I：

10.1016/0166-6851(91)90048-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A putative ATPase gene was cloned from Trypanosoma brucei genomic DNA. The length of the gene open reading frame is 3 033 bp, predicting a protein of about 110 kDa. The sequence of this protein shares 10 blocks of homology with other eukaryotic ATPases, including the putative phosphorylation site characteristic of P-ATPases. Its hydropathy profile reveals 8-10 potential membrane-spanning regions. While the amino acid sequence of the T. brucei ATPase shows only 25% overall homology with its counterpart from the related kinetoplastid protozoan Leishmania donovani, 49% sequence conservation is found when compared with the calcium-ATPase from rabbit sarcoplasmic reticulum. This gene is present in only one copy, localized in the large chromosome fraction. It is transcribed at a similar level in procyclic and bloodstream forms, as a 4.3-kb mRNA. Run-on assays suggest continuous transcription of the gene and flanking sequences over at least 10 kb, by a RNA polymerase sensitive to alpha-amanitin. Transcription inhibition by UV irradiation suggest that the ATPase gene is more than 4 kb downstream from its promoter.

引用

页码：241 / 251

页数：11

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