DEVELOPMENT OF A SIMPLE, SENSITIVE AND SPECIFIC BIOASSAY FOR INTERLEUKIN-1 BASED ON THE PROLIFERATION OF RPMI 1788 CELLS - COMPARISON WITH OTHER BIOASSAYS FOR IL-1

被引：26

作者：

VANDENABEELE, P ^{[1
]}

DECLERCQ, W ^{[1
]}

LIBERT, C ^{[1
]}

FIERS, W ^{[1
]}

机构：

[1] STATE UNIV GHENT,MOLEC BIOL LAB,LEDEGANCKSTR 35,B-9000 GHENT,BELGIUM

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 135卷 / 1-2期

关键词：

INTERLEUKIN-1; BIOASSAY; CYTOKINE;

D O I：

10.1016/0022-1759(90)90252-Q

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The IL-1-dependent proliferation of RPMI 1788, a human EBV-transformed cell line, was used to develop a biological assay system for IL-1. Preparations of rhIL-1-alpha and rhIL-1-beta, as well as rmIL-1-beta exhibited a specific biological activity (50% of the maximal response) between 5.8 x 10(8) and 8.6 x 10(8) U/mg. Remarkably, a 3-5 fold reduced specific biological activity was noticed for rm-IL-1-alpha, viz. 1.7 x 10(8) U/mg. The IL-1-dependent proliferation of RPMI 1788 cells was compared with other IL-1 test systems, such as the IL-1-mediated induction of IL-2 in EL4-NOB-1, LBRM-33-1A5 and thymocytes, and the IL-1-driven induction of cytotoxic activity by PC60 cells, the so-called CIA assay. The cytokine-dependent growth of RPMI 1788 cells is highly specific for IL-1, and no other cytokine tested induced a proliferative response. The presence of high concentrations of rmTNF, rhTNF or rhIL-6 did not interfere with the quantification of IL-1. Additionally, we evaluated the detection of IL-1 in the presence of mitogens, phorbol ester or calcium ionophore, as well as the determination of IL-1 in serum and PF samples of human and murine origin.

引用

页码：25 / 32

页数：8