PROTEIN-PHOSPHORYLATION IN ISOLATED-NUCLEI FROM ETIOLATED AVENA SEEDLINGS - EFFECTS OF RED FAR-RED LIGHT AND CHOLERA-TOXIN

被引：43

作者：

ROMERO, LC ^{[1
]}

BISWAL, B ^{[1
]}

SONG, PS ^{[1
]}

机构：

[1] UNIV NEBRASKA,DEPT CHEM,LINCOLN,NE 68588

来源：

FEBS LETTERS | 1991年 / 282卷 / 02期

关键词：

SIGNAL TRANSDUCTION; G-PROTEIN; PHYTOCHROME; NUCLEI; AVENA-SATIVA;

D O I：

10.1016/0014-5793(91)80510-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have studied the phosphorylation/dephosphorylation of several nuclear proteins is isolated nuclei from etiolated Avena seedlings as a function of red/far-red light. The effect of stimulatory (ADP-ribosylation by cholera toxin) or inhibitory (GDP-beta-S) conditions for GTP-binding proteins was also studied. Red or far-red light enhanced the phosphorylation level of 2 nuclear proteins with molecular masses of 75 and 60 kDa. The phosphorylation pattern was affected by the addition of cholera toxin or GDP-beta-S to the isolated nuclei. At least 2 proteins with molecular masses of 24 and 75 kDa cross-reacted by Western blot with GTP-binding protein antibodies.

引用

页码：347 / 350

页数：4

共 21 条

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