DETECTION OF HTLV-I PROVIRUS BY IN-SITU POLYMERASE CHAIN-REACTION IN MONONUCLEAR INFLAMMATORY CELLS IN SKELETAL-MUSCLE OF VIRAL CARRIERS WITH POLYMYOSITIS

被引：28

作者：

HIGUCHI, I

HASHIMOTO, K

KASHIO, N

IZUMO, S

INOSE, M

IZUMI, K

OHKUBO, R

NAKAGAWA, M

ARIMURA, K

OSAME, M

机构：

[1] Department of Internal Medicine, Kagoshima University, Kagoshima

来源：

MUSCLE & NERVE | 1995年 / 18卷 / 08期

关键词：

IN SITU PCR; HTLV-I; TAX; PROVIRUS; POLYMYOSITIS;

D O I：

10.1002/mus.880180809

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

We tested for HTLV-I proviral DNA in skeletal muscle from patients with polymyositis infected with HTLV-I using the in situ polymerase chain reaction. We found the HTLV-I provirus in some of the CD4-positive cells in HTLV-I-positive polymyositis cases but not in HTLV-I-negative polymyositis ones. We could not detect HTLV-I within the muscle fibers. We suggest that HTLV-I-associated polymyositis is not due to direct, persistent infection of the muscle fiber by the virus, but to a T-cell-mediated immunological process triggered by the HTLV-I-infected cells. (C) 1995 John Wiley and Sons, Inc.

引用

收藏

页码：854 / 858

页数：5

相关论文

共 23 条

[1] MONOCLONAL-ANTIBODY ANALYSIS OF MONONUCLEAR-CELLS IN MYOPATHIES .1. QUANTITATION OF SUBSETS ACCORDING TO DIAGNOSIS AND SITES OF ACCUMULATION AND DEMONSTRATION AND COUNTS OF MUSCLE-FIBERS INVADED BY T-CELLS [J].

ARAHATA, K ;

ENGEL, AG .

ANNALS OF NEUROLOGY, 1984, 16 (02) :193-208

[2] POLYMYOSITIS, DERMATOMYOSITIS, AND INCLUSION-BODY MYOSITIS [J].

DALAKAS, MC .

NEW ENGLAND JOURNAL OF MEDICINE, 1991, 325 (21) :1487-1498

[3] MASSIVE COVERT INFECTION OF HELPER T-LYMPHOCYTES AND MACROPHAGES BY HIV DURING THE INCUBATION PERIOD OF AIDS [J].

EMBRETSON, J ;

ZUPANCIC, M ;

RIBAS, JL ;

BURKE, A ;

RACZ, P ;

TENNERRACZ, K ;

HAASE, AT .

NATURE, 1993, 362 (6418) :359-362

[4] MONONUCLEAR-CELLS IN MYOPATHIES - QUANTITATION OF FUNCTIONALLY DISTINCT SUBSETS, RECOGNITION OF ANTIGEN-SPECIFIC CELL-MEDIATED CYTOTOXICITY IN SOME DISEASES, AND IMPLICATIONS FOR THE PATHOGENESIS OF THE DIFFERENT INFLAMMATORY MYOPATHIES [J].

ENGEL, AG ;

ARAHATA, K .

HUMAN PATHOLOGY, 1986, 17 (07) :704-721

[5] HUMAN T-CELL LEUKEMIA-VIRUS (HTLV-I) TRANSCRIPTS IN FRESH AND CULTURED-CELLS OF PATIENTS WITH ADULT T-CELL LEUKEMIA [J].

FRANCHINI, G ;

WONGSTAAL, F ;

GALLO, RC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (19) :6207-6211

[6] FAILURE TO DETECT HTLV-I BY INSITU HYBRIDIZATION IN THE BIOPSIED MUSCLES OF VIRAL CARRIERS WITH POLYMYOSITIS [J].

HIGUCHI, I ;

NERENBERG, M ;

YOSHIMINE, K ;

YOSHIDA, M ;

FUKUNAGA, H ;

TAJIMA, K ;

OSAME, M .

MUSCLE & NERVE, 1992, 15 (01) :43-47

[7] IMMUNOHISTOCHEMICAL CHARACTERISTICS OF POLYMYOSITIS IN PATIENTS WITH HTLV-I-ASSOCIATED MYELOPATHY AND HTLV-I CARRIERS [J].

HIGUCHI, I ;

MONTEMAYOR, ES ;

IZUMO, S ;

INOSE, M ;

OSAME, M .

MUSCLE & NERVE, 1993, 16 (05) :472-476

[8] INFECTION OF HUMAN-ENDOTHELIAL CELLS BY HUMAN T-LYMPHOTROPIC VIRUS TYPE-I [J].

HO, DD ;

ROTA, TR ;

HIRSCH, MS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (23) :7588-7590

[9] LYMPHOCYTE SUBSETS IN MUSCLE BIOPSIES OF HUMAN T-LYMPHOTROPIC VIRUS TYPE-I CARRIERS WITH POLYMYOSITIS [J].

IJICHI, T ;

HIGUCHI, I ;

FUKUNAGA, H ;

IZUMO, S ;

OSAME, M .

INTERNAL MEDICINE, 1992, 31 (08) :973-977

[10] PATHOLOGICAL-CHANGES IN SKELETAL-MUSCLE IN HTLV-I-ASSOCIATED MYELOPATHY [J].

INOSE, M ;

HIGUCHI, I ;

YOSHIMINE, K ;

SUEHARA, M ;

IZUMO, S ;

ARIMURA, K ;

OSAME, M .

JOURNAL OF THE NEUROLOGICAL SCIENCES, 1992, 110 (1-2) :73-78