LOSS OF MEDULLARY DENDRITIC CELLS IN THE THYMUS AFTER CYCLOSPORINE AND IRRADIATION

被引：24

作者：

BESCHORNER, WE

ARMAS, OA

机构：

[1] Immunopathology Unit, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore

来源：

CELLULAR IMMUNOLOGY | 1991年 / 132卷 / 02期

关键词：

D O I：

10.1016/0008-8749(91)90046-E

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Cyclosporine (CsA) induces a paradoxical graft-vs-host-like disease (GVHD) in syngeneic rat chimeras, providing the rat has a thymus and receives mediastinal irradiation. Here we evaluate the effect of CsA and irradiation on the relative abundance of thymic dendritic cells (DC). DC were identified with an immunoperoxidase stain for ED1 and were quantified by computerized planimetry. Normal young rats have scattered DC in the cortex and numerous DC in the medulla with a concentration at the cortico-medullary junction. Short-term CsA induces a marked loss of medullary DC (798 ± 126 cells/mm to 88 ± 103, P < 0.0001) and a modest loss of cortical DC (543 ± 55 to 330 ± 130, P < 0.01). While medullary DC normally recover promptly post-CsA, rats receiving mediastinal irradiation demonstrated minimal recovery post-CsA (P < 0.0003). The prolonged deficiency of medullary DC could represent an essential early step for loss of self-tolerance and sGVHD. © 1991.

引用

页码：505 / 514

页数：10

共 47 条

[21]

HATTORI A, 1987, AM J PATHOL, V28, P1

[22] THE HUMAN THYMIC MICROENVIRONMENT [J].

HAYNES, BF .

ADVANCES IN IMMUNOLOGY, 1984, 36 :87-142

[23] DEVELOPMENT OF GRAFT-VS-HOST DISEASE LIKE SYNDROME IN CYCLOSPORINE-TREATED RATS AFTER SYNGENEIC BONE-MARROW TRANSPLANTATION .1. DEVELOPMENT OF CYTO-TOXIC LYMPHOCYTES-T WITH APPARENT POLYCLONAL ANTI-IA SPECIFICITY, INCLUDING AUTOREACTIVITY [J].

HESS, AD ;

HORWITZ, L ;

BESCHORNER, WE ;

SANTOS, GW .

JOURNAL OF EXPERIMENTAL MEDICINE, 1985, 161 (04) :718-730

[24]

HESS AD, 1987, TRANSPLANT P, V19, P2683

[25]

HESS AD, 1990, J IMMUNOL, V145, P526

[26] DIFFERENTIAL EFFECT OF CYCLOSPORINE INVIVO ON THE DISTRIBUTION OF T-CELL SUBSETS IN THE THYMUS, SPLEEN, AND LYMPH-NODES [J].

HIRAMINE, C ;

HOJO, K ;

MATSUMOTO, H ;

KOSETO, M ;

ITOH, M .

TRANSPLANTATION, 1989, 47 (03) :499-503

[27] EFFECTS OF CYCLOSPORINE-A ON T-CELL DEVELOPMENT AND CLONAL DELETION [J].

JENKINS, MK ;

SCHWARTZ, RH ;

PARDOLL, DM .

SCIENCE, 1988, 241 (4873) :1655-1658

[28] INFLAMMATION-INDUCED ENDOTHELIAL-CELL ADHESION TO LYMPHOCYTES, NEUTROPHILS, AND MONOCYTES - ROLE OF HOMING RECEPTORS AND OTHER ADHESION MOLECULES [J].

JUTILA, MA ;

BERG, EL ;

KISHIMOTO, TK ;

PICKER, LJ ;

BARGATZE, RF ;

BISHOP, DK ;

OROSZ, CG ;

WU, NW ;

BUTCHER, EC .

TRANSPLANTATION, 1989, 48 (05) :727-731

[29] T-CELL TOLERANCE BY CLONAL ELIMINATION IN THE THYMUS [J].

KAPPLER, JW ;

ROEHM, N ;

MARRACK, P .

CELL, 1987, 49 (02) :273-280

[30] ABSENCE OF THE LYT-2-,L3T4+ LINEAGE OF T-CELLS IN MICE TREATED NEONATALLY WITH ANTI-I-A CORRELATES WITH ABSENCE OF INTRATHYMIC I-A-BEARING ANTIGEN-PRESENTING CELL-FUNCTION [J].

KRUISBEEK, AM ;

MOND, JJ ;

FOWLKES, BJ ;

CARMEN, JA ;

BRIDGES, S ;

LONGO, DL .

JOURNAL OF EXPERIMENTAL MEDICINE, 1985, 161 (05) :1029-1047

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