SEPARATION OF ENANTIOMERS ON A PEPSIN-BONDED COLUMN

被引：43

作者：

HAGINAKA, J

MIYANO, Y

SAIZEN, Y

SEYAMA, C

MURASHIMA, T

机构：

[1] Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, 663, 11-68, Koshien Kyuban-cho

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 708卷 / 01期

关键词：

D O I：

10.1016/0021-9673(95)00357-S

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Commercial crystalline pepsin from porcine stomach mucosa was characterized by high-performance capillary electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. These results suggest that the average molecular mass of the pepsin is about 34 900, and that it contains heterogeneity due to the autodigestion. The pepsin was covalently bound to porous aminopropylsilica materials using an N,N'-disuccinimidyl carbonate reaction. By using a mixture of phosphate buffer and organic modifier as an eluent, basic and uncharged enantiomers were resolved, while no resolution of acidic enantiomers was observed.

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页码：161 / 168

页数：8

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