MOLECULAR-ORGANIZATION OF THE DECAPENTAPLEGIC GENE IN DROSOPHILA-MELANOGASTER

被引：180

作者：

STJOHNSTON, RD ^{[1
]}

HOFFMANN, FM ^{[1
]}

BLACKMAN, RK ^{[1
]}

SEGAL, D ^{[1
]}

GRIMAILA, R ^{[1
]}

PADGETT, RW ^{[1
]}

IRICK, HA ^{[1
]}

GELBART, WM ^{[1
]}

机构：

[1] HARVARD UNIV,DEPT CELLULAR & DEV BIOL,CAMBRIDGE,MA 02138

来源：

GENES & DEVELOPMENT | 1990年 / 4卷 / 07期

关键词：

Dpp gene; Drosophila; Family; Imaginal disks; Pattern formation; TGF-β;

D O I：

10.1101/gad.4.7.1114

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The decapentaplegic (dpp) locus of Drosopbila melanogaster is a >55 kb genetic unit required for proper pattern formation during the embryonic and imaginai development of the organism. We have proposed that these morphogenetic functions result from the action of a secreted transforming growth factor-β (TGF-β)-related protein product encoded by dpp. In this paper we localize 60 mutations on the molecular map of dpp. The positions of these mutations cluster according to phenotypic class, identifying the locations of specific dpp functions. By Northern and cDNA analysis, we characterize five overlapping dpp transcripts. On the basis of the locations of the overlaps relative to a previously sequenced cDNA, it is likely that these transcripts all encode similar or identical polypeptides. We propose that the bulk of dpp DNA consists of extensive arrays of cis-regulatory information. The large (>25-kb) 3′ cis-regulatory region represents a novel feature of dpp gene organization.

引用

页码：1114 / 1127

页数：14

共 49 条

[11] FUNCTIONAL CDNA LIBRARIES FROM DROSOPHILA EMBRYOS [J].

BROWN, NH ;

KAFATOS, FC .

JOURNAL OF MOLECULAR BIOLOGY, 1988, 203 (02) :425-437

[12] LOCALIZED CELL-DEATH CAUSED BY MUTATIONS IN A DROSOPHILA GENE CODING FOR A TRANSFORMING GROWTH FACTOR-BETA HOMOLOG [J].

BRYANT, PJ .

DEVELOPMENTAL BIOLOGY, 1988, 128 (02) :386-395

[13] THE ROLE OF THE HAIRY GENE DURING DROSOPHILA MORPHOGENESIS - STRIPES IN IMAGINAL DISKS [J].

CARROLL, SB ;

WHYTE, JS .

GENES & DEVELOPMENT, 1989, 3 (06) :905-916

[14] ISOLATION OF THE BOVINE AND HUMAN GENES FOR MULLERIAN INHIBITING SUBSTANCE AND EXPRESSION OF THE HUMAN-GENE IN ANIMAL-CELLS [J].

CATE, RL ;

MATTALIANO, RJ ;

HESSION, C ;

TIZARD, R ;

FARBER, NM ;

CHEUNG, A ;

NINFA, EG ;

FREY, AZ ;

GASH, DJ ;

CHOW, EP ;

FISHER, RA ;

BERTONIS, JM ;

TORRES, G ;

WALLNER, BP ;

RAMACHANDRAN, KL ;

RAGIN, RC ;

MANGANARO, TF ;

MACLAUGHLIN, DT ;

DONAHOE, PK .

CELL, 1986, 45 (05) :685-698

[15] HUMAN TRANSFORMING GROWTH FACTOR-BETA COMPLEMENTARY-DNA SEQUENCE AND EXPRESSION IN NORMAL AND TRANSFORMED-CELLS [J].

DERYNCK, R ;

JARRETT, JA ;

CHEN, EY ;

EATON, DH ;

BELL, JR ;

ASSOIAN, RK ;

ROBERTS, AB ;

SPORN, MB ;

GOEDDEL, DV .

NATURE, 1985, 316 (6030) :701-705

[16]

DINOCERA PP, 1983, J MOL BIOL, V168, P715

[17] TRANSCRIPTS ENCODED BY A HOMOEO BOX GENE ARE RESTRICTED TO DORSAL TISSUES OF DROSOPHILA EMBRYOS [J].

DOYLE, HJ ;

HARDING, K ;

HOEY, T ;

LEVINE, M .

NATURE, 1986, 323 (6083) :76-79

[18] THE DECAPENTAPLEGIC GENE-COMPLEX IN DROSOPHILA-MELANOGASTER [J].

GELBART, WM ;

IRISH, VF ;

STJOHNSTON, RD ;

HOFFMANN, FM ;

BLACKMAN, RK ;

SEGAL, D ;

POSAKONY, LM ;

GRIMAILA, R .

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1985, 50 :119-125

[19] IDENTIFICATION IN TRANSGENIC ANIMALS OF THE DROSOPHILA-DECAPENTAPLEGIC SEQUENCES REQUIRED FOR EMBRYONIC DORSAL PATTERN-FORMATION [J].

HOFFMANN, FM ;

GOODMAN, W .

GENES & DEVELOPMENT, 1987, 1 (06) :615-625

[20] REGULATION AND PRODUCTS OF THE UBX DOMAIN OF THE BITHORAX COMPLEX [J].

HOGNESS, DS ;

LIPSHITZ, HD ;

BEACHY, PA ;

PEATTIE, DA ;

SAINT, RB ;

GOLDSCHMIDTCLERMONT, M ;

HARTE, PJ ;

GAVIS, ER ;

HELFAND, SL .

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1985, 50 :181-194

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