SEPARATION AND PARTIAL CHARACTERIZATION OF 2 GALACTOSYLTRANSFERASE ISOFORMS FROM MALIGNANT ASCITIC FLUID

被引：6

作者：

BOYLE, FA

COOK, ND

PETERS, TJ

机构：

来源：

CLINICA CHIMICA ACTA | 1988年 / 171卷 / 2-3期

关键词：

D O I：

10.1016/0009-8981(88)90144-1

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Galactosyltransferase was isolated from human malignant ascites by ammonium sulphate precipitation, .alpha.-lactalbumin affinity chromatography and by the removal of contaminating immunoglobulins with protein A chromatography. Two isoforms of galactosyltransferase were separated by DEAE chromatography, and partially characterized by gel filtration, non-denaturing polyacrylamide gel electrophoresis, concanavalin A binding and enzyme kinetics. The two forms isolated were compared with the isoenzymes previously described and the biochemical basis for the difference between the two forms discussed.

引用

页码：187 / 196

页数：10

共 24 条

[1] ANALYTICAL STUDY OF MICROSOMES AND ISOLATED SUBCELLULAR MEMBRANES FROM RAT-LIVER .1. BIOCHEMICAL METHODS [J].