EFFECT OF PENTOXIFYLLINE ON SPERM MOVEMENT CHARACTERISTICS IN NORMOZOOSPERMIC AND ASTHENOZOOSPERMIC SPECIMENS

被引：110

作者：

TESARIK, J

THEBAULT, A

TESTART, J

机构：

[1] Centre for Reproductive Biology and Medicine, American Hospital of Paris, 92202 Neuilly sur Seine, 63 boulevard Victor Hugo

[2] INSERM, Unit 355, 92140 Clamart

来源：

HUMAN REPRODUCTION | 1992年 / 7卷 / 09期

关键词：

AUTOMATED SPERM ANALYSIS; HYPERACTIVATION; PENTOXIFYLLINE; SPERM MOTION IMPROVEMENT; VELOCITY;

D O I：

10.1093/oxfordjournals.humrep.a137837

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Preliminary evidence has suggested that the phosphodiesterase inhibitor pentoxifylline augments the fertilizing potential of asthenozoospermic sperm samples, presumably by improving sperm movement. Here, we used computer-assisted sperm movement analysis to compare the effects of pentoxifylline in normozoospermic and asthenozoospermic specimens. The study focused on the following issues: the changes in individual movement characteristics in response to pentoxifylline, the rapidity of the response, the effect of sperm capacitation on the response, the persistence of the response after drug removal and the variability of responses among asthenozoospermic individuals. Data obtained show that (i) pentoxifylline increases the curvilinear velocity, path velocity, straight-line velocity, lateral head displacement, beat cross frequency and sperm hyperactivation in both normozoospermic and asthenozoospermic specimens, (ii) pentoxifylline does not modify the percentage of motile spermatozoa, (iii) the pentoxifylline effect reaches a maximum within 10 min of treatment in fresh semen as well as in capacitated sperm suspensions and persists for at least 2 h after drug removal and (iv) pentoxifylline improves the movement characteristics in most asthenozoospermic individuals. Results are discussed with regard to methods of therapeutic application of pentoxifylline as an enhancer of sperm movement in assisted reproductive technology.

引用

页码：1257 / 1263

页数：7

共 29 条

[21]

Tamblyn T.M., First N.L., Caffeine-stimulated ATP- reactivated motility in a detergent-treated bovine sperm model, Arch. Biochem. Biophys, 181, pp. 208-215, (1977)

[22]

Tash J.S., Means A.R., Cyclic adenosine 3', 5'-monophos- phale, calcium and protein phosphorylation in flagellar motility, Biol. Reprod, 28, pp. 75-104, (1983)

[23]

Tesarik J., Testart J., Human sperm-egg interactions and their disorders: Implications in the management of infertility, Hum. Reprod, 4, pp. 729-741, (1989)

[24]

Tesarik J., Drahorad J., Testart J., Mendoza C., Acrosin Activation Follows Its Surface Exposure and Precedes Membrane Fusion in Human Sperm Acrosome Reaction. Development, 110, (1990)

[25]

Tesarik J., Mendoza Oltras C., Testart J., Effect of the human cumulus oophorus on movement characteristics of human capacitated spermatozoa, J. Reprod. Fertil, 88, (1990)

[26]

Ward A., Clissold S.P., Pentoxifylline: A review of its pharmacodynamic and pharmacokinetic properties, and its therapeutic efficiency, Drugs, 34, pp. 50-60, (1987)

[27]

Laboratory Manual for the Examination of Human Semen and Semen-Cervical Mucus Interaction, pp. 1-67, (1987)

[28]

Yovich J.M., Edirisinghe W.R., Cummins M., Yovich J.L., Preliminary results using pentoxifylline in a pronuclear stage tubal transfer (PROST) program for severe male factor infertility, Fertil. Steril, 50, pp. 179-181, (1988)

[29]

Yovich J.M., Edirisinghe W.R., Cummins M., Yovich J.L., Influence of pentoxifylline in severe male factor infertility, Fertil. Steril, 53, (1990)

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