A SIMPLE AND FAST METHOD TO DETERMINE AND QUANTIFY URINARY CREATININE

被引：40

作者：

SHI, HL ^{[1
]}

MA, YQ ^{[1
]}

MA, YF ^{[1
]}

机构：

[1] NE MISSOURI STATE UNIV,DIV SCI,KIRKSVILLE,MO 63501

来源：

ANALYTICA CHIMICA ACTA | 1995年 / 312卷 / 01期

关键词：

HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; ELECTROPHORESIS; CREATININE; URINE;

D O I：

10.1016/0003-2670(95)00208-H

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and fast method for the determination of urinary creatinine by high-performance capillary electrophoresis (HPCE) has been developed. The urine samples were diluted 50 fold before they were injected onto an HPCE column for analysis. With this technique, the urinary creatinine level can be determined within 4 min. The interference from urea in urine has been totally eliminated. The reproducibility and recovery of the technique have been studied and it was proven that the technique is satisfactory for quantitative determination of urine creatinine. The results obtained from this technique have been compared with results obtained by high-performance liquid chromatography, and the results from both techniques agreed quite well (correlation coefficient = 0.993). The linear range of the technique was over two orders of magnitude (0.1-11 mg/dl) which covers the creatinine concentration in human urine after 50 fold dilution. The detection limit for creatinine is 0.04 mg/dl with a signal-to-noise ratio of 3.

引用

页码：79 / 83

页数：5

共 13 条

[11]

WERNER ER, 1987, CLIN CHEM, V33, P62

[12] DETERMINATION OF TOTAL POLYAMINES IN TUMOR-CELLS BY HIGH-PERFORMANCE CAPILLARY ZONE ELECTROPHORESIS WITH INDIRECT PHOTOMETRIC DETECTION [J].