DETERMINATION OF 6-MERCAPTOPURINE IN ACUTE LYMPHOBLASTIC-LEUKEMIA PATIENTS PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：15

作者：

KATO, Y

MATSUSHITA, T

YOKOYAMA, T

MOHRI, K

机构：

[1] MEIJI COLL PHARM,DEPT BIOPHARM,TOKYO 154,JAPAN

[2] NATL MED CTR HOSP,DEPT PEDIAT,SHINJUKU KU,TOKYO 162,JAPAN

来源：

THERAPEUTIC DRUG MONITORING | 1991年 / 13卷 / 03期

关键词：

6-MERCAPTOPURINE; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; SOLID PHASE EXTRACTION; ACUTE LYMPHOBLASTIC LEUKEMIA;

D O I：

10.1097/00007691-199105000-00006

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A simple, selective, sensitive, and rapid high-performance liquid chromatography (HPLC) method is described for the quantitation of 6-mercaptopurine (6-MP) in plasma of patients with acute lymphoblastic leukemia (ALL). Pharmacokinetic data are presented for seven children with ALL receiving 6-MP therapy. A sensitivity of greater-than-or-equal-to 2 ng/ml in plasma was achieved on a reverse-phase octadecylsilane column using an HPLC system following a cleanup step with a solid-phase extraction cartridge. The chromatogram was monitored at 325 nm. Analytical recovery of 6-MP was 90%. The coefficients of variation for intra- and interday variabilities were 2.51 and 4.23%. This assay method is clinically useful for pharmacokinetic studies of 6-MP in ALL patients.

引用

收藏

页码：220 / 225

页数：6

相关论文

共 23 条

[1] GAS-CHROMATOGRAPHIC METHOD FOR MEASURING 6-MERCAPTOPURINE IN SERUM [J].

BAILEY, DG ;

WILSON, TW ;

JOHNSON, GE .

JOURNAL OF CHROMATOGRAPHY, 1975, 111 (02) :305-311

[2] CLINICAL EVALUATION OF A NEW ANTIMETABOLITE, 6-MERCAPTOPURINE, IN THE TREATMENT OF LEUKEMIA AND ALLIED DISEASES [J].

BURCHENAL, JH ;

MURPHY, ML ;

ELLISON, RR ;

SYKES, MP ;

TAN, TC ;

LEONE, LA ;

KARNOFSKY, DA ;

CRAVER, LF ;

DARGEON, HW ;

RHOADS, CP .

BLOOD, 1953, 8 (11) :965-999

[3] A NOVEL METHOD FOR THE QUANTITATION OF 6-MERCAPTOPURINE IN HUMAN-PLASMA USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION [J].

BURTON, NK ;

AHERNE, GW ;

MARKS, V .

JOURNAL OF CHROMATOGRAPHY, 1984, 309 (02) :409-414

[4]

CALABRESI P, 1985, PHARMACOL BASIS THER, P1275

[5] DETERMINATION OF 6-MERCAPTOPURINE AND AZATHIOPRINE IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

DING, TL ;

BENET, LZ .

JOURNAL OF CHROMATOGRAPHY, 1979, 163 (03) :281-288

[6] 6-MERCAPTOPURINE PLASMA-LEVELS IN CHILDREN WITH ACUTE LYMPHOBLASTIC-LEUKEMIA - RELATION TO RELAPSE RISK AND MYELOTOXICITY [J].

HAYDER, S ;

LAFOLIE, P ;

BJORK, O ;

PETERSON, C .

THERAPEUTIC DRUG MONITORING, 1989, 11 (06) :617-622

[7] SYSTEMIC EXPOSURE TO MERCAPTOPURINE AS A PROGNOSTIC FACTOR IN ACUTE LYMPHOCYTIC-LEUKEMIA IN CHILDREN [J].

KOREN, G ;

FERRAZINI, G ;

SULH, H ;

LANGEVIN, AM ;

KAPELUSHNIK, J ;

KLEIN, J ;

GIESBRECHT, E ;

SOLDIN, S ;

GREENBERG, M .

NEW ENGLAND JOURNAL OF MEDICINE, 1990, 323 (01) :17-21

[8] LARGE INTERINDIVIDUAL VARIATIONS IN THE PHARMACOKINETICS OF ORAL 6-MERCAPTOPURINE IN MAINTENANCE THERAPY OF CHILDREN WITH ACUTE-LEUKEMIA AND NON-HODGKIN LYMPHOMA [J].

LAFOLIE, P ;

HAYDER, S ;

BJORK, O ;

AHSTROM, L ;

LILIEMARK, J ;

PETERSON, C .

ACTA PAEDIATRICA SCANDINAVICA, 1986, 75 (05) :797-803

[9] ASSAY OF 6-MERCAPTOPURINE IN HUMAN-PLASMA [J].

LENNARD, L .

JOURNAL OF CHROMATOGRAPHY, 1985, 345 (02) :441-446

[10] DETERMINATION OF PLASMA AZATHIOPRINE AND 6-MERCAPTOPURINE IN PATIENTS WITH RHEUMATOID-ARTHRITIS TREATED WITH ORAL AZATHIOPRINE [J].

LILIEMARK, J ;

PETTERSSON, B ;

LAFOLIE, P ;

ZWEIG, T ;

PETERSON, C .

THERAPEUTIC DRUG MONITORING, 1990, 12 (04) :339-343