DESIGNER DYES - BIOMIMETIC LIGANDS FOR THE PURIFICATION OF PHARMACEUTICAL PROTEINS BY AFFINITY-CHROMATOGRAPHY

被引：78

作者：

LOWE, CR

BURTON, SJ

BURTON, NP

ALDERTON, WK

PITTS, JM

THOMAS, JA

机构：

[1] AFFIN CHROMATOG LTD,CAMBRIDGE CB3 0JX,ENGLAND

[2] UNIV WARWICK,DEPT BIOL SCI,COVENTRY CV4 7AL,W MIDLANDS,ENGLAND

来源：

TRENDS IN BIOTECHNOLOGY | 1992年 / 10卷 / 12期

关键词：

D O I：

10.1016/0167-7799(92)90294-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Affinity chromatography has been extensively refined over the past few years to meet the more stringent criteria being placed on recombinant proteins as therapeutic products. New developments in the design of selective and stable ligands for affinity chromatography are establishing the technique as a routine tool in process-scale protein purification. Exploitation of sophisticated molecular modelling techniques in conjunction with binding and crystallographic studies has permitted the design of new, highly selective 'biomimetic' ligands for the target proteins.

引用

页码：442 / 448

页数：7

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