COEXISTENCE OF 2 CALSEQUESTRIN ISOFORMS IN RABBIT SLOW-TWITCH SKELETAL-MUSCLE FIBERS

被引：36

作者：

BIRAL, D

VOLPE, P

DAMIANI, E

MARGRETH, A

机构：

[1] Centro di Studio per la Biologia e la Fisiopatologia Muscolare, CNR, Istituto di Patologia Generale dell'Università di Padova, 35121 Padova

来源：

FEBS LETTERS | 1992年 / 299卷 / 02期

关键词：

SKELETAL MUSCLE; SARCOPLASMIC RETICULUM; CALSEQUESTRIN; CA-2+;

D O I：

10.1016/0014-5793(92)80241-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cardiac and skeletal muscle isoforms of calsequestrin (CS), the low affinity, high capacity Ca2+ binding protein localized in the lumen of sarcoplasmic reticulum, are the products of two different genes (Fliegel, L., Leberer, E., Green, N.M. and MacLennan, D.H. (1982) FEBS Lett. 242, 297-300), and can be both purified from slow-twitch skeletal muscle of the rabbit (Damiani, E., Volpe, P. and Margreth, A. (1990) J. Muscle Res. Cell Motil. 11, 522-530). Here we show that both CS isoforms coexist in slow-twitch muscle fibers as indicated by indirect immunofluorescent staining of cryosections with affinity-purified antibodies specific for each CS isoform.

引用

页码：175 / 178

页数：4

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