PHOSPHORYLATION OF SYNTHETIC VIMENTIN PEPTIDES BY CDC2 KINASE

被引：20

作者：

ANDO, S

TSUJIMURA, K

MATSUOKA, Y

TOKUI, T

HISANAGA, S

OKUMURA, E

UCHIYAMA, M

KISHIMOTO, T

YASUDA, H

INAGAKI, M

机构：

[1] AICHI CANC CTR, RES INST, EXPTL RADIOL LAB, CHIKUSA KU, NAGOYA, AICHI 464, JAPAN

[2] TOKYO METROPOLITAN GERIATR HOSP & INST GERONTOL, DEPT NEUROPHYSIOL, ITABASHI KU, TOKYO 173, JAPAN

[3] TOKYO INST TECHNOL, FAC BIOSCI, CELL & DEV BIOL LAB, MIDORI KU, YOKOHAMA, KANAGAWA 227, JAPAN

[4] KANAZAWA UNIV, FAC PHARMACEUT SCI, DIV BIOL, KANAZAWA, ISHIKAWA 920, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 195卷 / 02期

关键词：

D O I：

10.1006/bbrc.1993.2121

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Synthetic peptide representing the site Ser-41 in vimentin, Leu-Gly-Ser41-A1a42-Leu-Arg44-Arg-Arg-N H2, and its analogs in which Ala-42 was replaced by various amino acids were tested as substrates for cdc2 kinase. Among them, the analog containing sarcosine as well as proline was an excellent substrate. The result suggests that the N-substituted structure of proline immediately following the site is important for cdc2 kinase phosphorylation. Replacement of Ala-42 by polar amino acids, especially lysine, had negative effects on peptide phosphorylation. The peptides in this study were also assayed with another type of proline-directed protein kinase, tau protein kinase II. The substrate specificity differed essentially from that of cdc2 kinase. © 1993 Academic Press, Inc.

引用

页码：837 / 843

页数：7