FUSOGENIC SEGMENTS OF BOVINE LEUKEMIA-VIRUS AND SIMIAN IMMUNODEFICIENCY VIRUS ARE INTERCHANGEABLE AND MEDIATE FUSION BY MEANS OF OBLIQUE INSERTION IN THE LIPID BILAYER OF THEIR TARGET-CELLS

被引：98

作者：

VONECHE, V

PORTELLE, D

KETTMANN, R

WILLEMS, L

LIMBACH, K

PAOLETTI, E

RUYSSCHAERT, JM

BURNY, A

BRASSEUR, R

机构：

[1] STATE UNIV LIEGE, B-4020 LIEGE, BELGIUM

[2] VIROGENET CORP, TROY, NY 12180 USA

[3] UNIV BRUSSELS, FAC SCI, MACROMOLEC INTERFACES LAB, B-1050 BRUSSELS, BELGIUM

[4] UNIV BRUSSELS, DEPT MOLEC BIOL, B-1640 RHODE ST GENESE, BELGIUM

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 09期

关键词：

BOVINE LEUKEMIA VIRUS ENVELOPE; FUSOGENIC SEGMENT OF BOVINE LEUKEMIA VIRUS AND SIMIAN IMMUNODEFICIENCY VIRUS; CONFORMATIONAL ANALYSIS;

D O I：

10.1073/pnas.89.9.3810

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Modified bovine leukemia virus (BLV) glycoproteins were expressed by using vaccinia virus recombinants, and their fusogenic capacities were examined by a syncytia-formation assay. This analysis indicates that (i) both BLV envelope glycoproteins gp51 and gp30 are necessary for cell fusion; (ii) insertion of the N-terminal segment of gp30 (fusion peptide) into the lipid bilayer in an oblique orientation, as predicted by computer conformational analysis, results in fusogenic capacities higher than insertion in a perpendicular or parallel orientation; and (iii) replacement of the BLV fusion peptide with its simian immunodeficiency virus counterpart does not modify the fusogenic capacity of the BLV glycoprotein.

引用

页码：3810 / 3814

页数：5

共 30 条

[1] IDENTIFICATION OF THE FUSION PEPTIDE OF PRIMATE IMMUNODEFICIENCY VIRUSES [J].