INTERLEUKIN-6 PRODUCTION BY RAT GRANULOSA-CELLS INVITRO - EFFECTS OF CYTOKINES, FOLLICLE-STIMULATING-HORMONE, AND CYCLIC 3',5'-ADENOSINE-MONOPHOSPHATE

被引:58
作者
GOROSPE, WC [1 ]
SPANGELO, BL [1 ]
机构
[1] MED UNIV S CAROLINA,DEPT PHYSIOL,CHARLESTON,SC 29425
关键词
D O I
10.1095/biolreprod48.3.538
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
In the present study we examined the influence of FSH as well as a number of well-established cytokines on interleukin (IL)-6 by rat granulosa cells in culture. Increasing concentrations of FSH, IL-1alpha, IL-1beta, tumor necrosis factor alpha (TNFalpha), and lipopolysaccharide (LPS) were incubated for 48 h with undifferentiated granulosa cells obtained from diethylstilbestrol-primed immature rats. The results demonstrate that FSH, IL-1alpha, IL-1beta, and LPS, but not TNFalpha, caused significant concentration-dependent increases in IL-6 release. We also examined the effects of dibutyryl-cAMP, forskolin, and 3-isobutyl-1-methyl-xanthine (IBMX) on IL-6 release by granulosa cells. Each of these agents caused a significant concentration-dependent increase in IL-6 production by granulosa cells in either the absence or presence of FSH. Taken together, these results show that the granulosa cell is not only a likely source of IL-6 but that the release of IL-6 can be regulated. Moreover, evidence suggests that cAMP may serve as a second messenger for the stimulated secretion of IL-6 by undifferentiated granulosa cells.
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页码:538 / 543
页数:6
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