THE PREDICTIVE VALUE OF BASAL FOLLICLE-STIMULATING AND GROWTH-HORMONE LEVELS AS DETERMINED BY IMMUNOFLUOROMETRY DURING ASSISTED REPRODUCTION

被引：16

作者：

HUYSER, C

FOURIE, FLR

PENTZ, J

HURTER, P

机构：

[1] Centre for Fertility Studies, Department of Obstetrics and Gynaecology, University of Pretoria, Pretoria, 0002

[2] Department of Chemical Pathology, University of Pretoria, Pretoria

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1995年 / 12卷 / 04期

关键词：

ASSISTED REPRODUCTION; AGE; IMMUNOFLUOROMETRIC ASSAY; FOLLICLE STIMULATING HORMONE; GROWTH HORMONE;

D O I：

10.1007/BF02212926

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Purpose: Our purpose was (a) to investigate relationships of baseline endocrine serum levels with selected assisted reproduction (AR) parameters and (b) to evaluate the clinical applicability of an immunofluorometric assay (IFMA) as an alternative to a radioimmunoassay (RIA). Methods: Basal endocrine values (analyzed by RIA-rFSH, rLH, and rE(2); analyzed by IFMA-fFSH, fLH, and fGH) were determined for female patients (n = 142) preceding ovarian stimulation for AR. Results: Specific AR parameters correlated significantly with RIA- and IFMA-determined FSH levels, although IFMA correlations consistently exceeds that of RIA. Cutoff values of fFSH greater than or equal to 11.68 IU/L or rFSH greater than or equal to 15.0 IU/L indicated a poor response. The high-basal fFSH group was older (34 vs 31 years; P = 0.0334) and yielded fewer oocytes (2.9 vs 4.6 oocytes; P = 0.0018) than the low-basal fFSH group (< 11.68 IU/L). Lower cumulative embryo scores and conception rates were also associated with the high-fFSH group, compared to the low-fFSH group. Conclusions: This study confirms the negative impact of elevated basal FSH levels on AR. Basal LH, GH, and E(2) levels are, in comparison to baseline FSH levels, unsuitable indices for estimating ovarian responsiveness. IFMA can be regarded as an alternative to RIA in both clinical and research laboratories.

引用

页码：244 / 251

页数：8

共 39 条

[21]

Haavisto A-M, Dunkel L., Petterson K., Huhtaniemi I., LH Measurements by in vitro bioassay and a highly sensitive immunofluorometric assay improve the distinction between boys with constitutional delay of puberty and hypogonadotropic hypogonadism, Pediatr Res, 27, pp. 211-214, (1990)

[22]

Apter D., Cacciatore B., Alfthan H., Stenman U-H, Serum luteinizing hormone concentrations increase 100-fold in females from 7 years of age to adulthood, as measured by time-resolved immunofluorometric assay, J Clin Endocrinol Metab, 68, pp. 53-57, (1989)

[23]

Dunkel L., Alfthan H., Stenman U-H, Tapanainen P., Perheentupa J., Pulsatile secretion of LH and FSH in prepubertal and early pubertal boys revealed by ultrasensitive time-resolved immunofluorometric assays, Pediatr Res, 27, pp. 215-219, (1990)

[24]

Visser D.S., Fourie, Kruger H.F., Multiple attempts at embryo transfer: Effect on pregnancy outcome in an in vitro fertilization and embryo transfer program, J Assist Reprod Genet, 10, pp. 37-43, (1993)

[25]

Visser D.S., Fourie, The applicability of the cumulative embryo score system for embryo selection and quality control in an in-vitro fertilization/embryo transfer programme, Hum Reprod, 8, pp. 1719-1722, (1993)

[26]

Griner P.F., Mayewski R.J., Mushlin A.I., Greenland P., Selection and interpretation of diagnostic tests and procedures, Ann Intern Med, 94, pp. 553-587, (1981)

[27]

Metz C.E., Basic principals of ROC analysis, Semin Nucl Med, 8, pp. 283-298, (1978)

[28]

Zweig M.H., Campbell G., Receiver-operating characteristic (ROC) plots: A fundamental evaluation tool in clinical medicine, Clin Chem, 39, pp. 561-577, (1993)

[29]

Jacobs S.L., Metzger D.A., Dodson W.C., Haney A.F., Effect of age on response to human menopausal gonadotropin stimulation, J Clin Endocrinol Metab, 71, pp. 1525-1530, (1990)

[30]

Kruger T.F., Acosta A.A., Simmons K.F., Swanson R.J., Matta J.F., Oehninger S., Predictive value of abnormal sperm morphology in in vitro fertilization, Fertil Steril, 49, pp. 112-117, (1988)

← 1 2 3 4 →