RECENT ADVANCES IN THE POLYMERASE CHAIN-REACTION

被引：818

作者：

ERLICH, HA ^{[1
]}

GELFAND, D ^{[1
]}

SNINSKY, JJ ^{[1
]}

机构：

[1] CETUS CORP,DEPT INFECT DIS,EMERYVILLE,CA 94608

来源：

SCIENCE | 1991年 / 252卷 / 5013期

关键词：

D O I：

10.1126/science.2047872

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The polymerase chain reaction (PCR) has dramatically altered how molecular studies are conducted as well as what questions can be asked. In addition to simplifying molecular tasks typically carried out with the use of recombinant DNA technology, PCR has allowed a spectrum of advances ranging from the identification of novel genes and pathogens to the quantitation of characterized nucleotide sequences. PCR can provide insights into the intricacies of single cells as well as the evolution of species. Some recent developments in instrumentation, methodology, and applications of the PCR are presented in this review.

引用

页码：1643 / 1651

页数：9

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