PROTEINS OF TRANSCRIPTION FACTOR ISGF-3 - ONE GENE ENCODES THE 91-KDA AND 84-KDA ISGF-3 PROTEINS THAT ARE ACTIVATED BY INTERFERON-ALPHA

被引：586

作者：

SCHINDLER, C

FU, XY

IMPROTA, T

AEBERSOLD, R

DARNELL, JE

机构：

[1] UNIV BRITISH COLUMBIA,BIOMED RES CTR,VANCOUVER V6T 1W5,BC,CANADA

[2] UNIV BRITISH COLUMBIA,DEPT BIOCHEM,VANCOUVER V6T 1W5,BC,CANADA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 16期

关键词：

D O I：

10.1073/pnas.89.16.7836

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

ISGF-3 is an interferon-dependent positive-acting transcription factor that is cytoplasmically activated, possibly through direct interaction with the interferon receptor. The factor has been purified, its component proteins have been separated, and its peptide sequences have been obtained. From the sequences, degenerate oligonucleotide probes were constructed to screen for cDNA clones. Sequencing of the selected clones shows that the 91- and 84-kDa components represent two forms of a previously unknown (to our knowledge) protein. Several antibodies raised against these proteins prove that they indeed do encode protein components of ISGF-3. This work provides reagents to explore the modification of this cytoplasmically activated transcription factor.

引用

页码：7836 / 7839

页数：4

共 17 条

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