A COMPARISON OF AMIDE AND UREIDE NITROGEN-SOURCES IN TISSUE-CULTURE OF TREE LEGUME PROSOPIS-ALBA CLONE B2 V-50

被引:8
作者
GREEN, B [1 ]
TABONE, T [1 ]
FELKER, P [1 ]
机构
[1] TEXAS A&I UNIV,COLL AGR,CAESAR KLEBERG WILDLIFE RES INST,CTR SEMIARID FOREST RESOURCES,KINGSVILLE,TX 78363
关键词
amide; in vitro culture; mesquite; Prosopis alba; shoot development; ureide;
D O I
10.1007/BF00034497
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The effects of medium nitrogen sources on the recalcitrant nature of Prosopis alba clone B2V50 in tissue culture were compared involving shoot development using axillary bud explants from 2 to 4-year-old greenhouse-grown trees. A significant difference (P<0.05) was found between the amino acids aspartic acid and glutamic acid and their corresponding amide-containing compounds asparagine and glutamine. A comparison between amide and ureide nitrogen sources showed that allantoin, a ureide, was an acceptable replacement for asparagine or glutamine. Allantoin, asparagine, and glutamine could be used as the sole nitrogen sources. Allantoin at a concentration of 20 mM was adopted for use in future research. Although shoots were consistently induced, all explants showed complete shoot-tip necrosis after 12 weeks of in vitro culture. © 1990 Kluwer Academic Publishers.
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页码:83 / 86
页数:4
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