THE STRUCTURAL STABILITY OF AN EXPRESSION PLASMID BEARING A HETEROLOGOUS CLONED GENE DEPENDS ON WHETHER THIS GENE IS EXPRESSED OR NOT

被引：7

作者：

KAPRALEK, F

JECMEN, P

机构：

[1] Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague 6, 166 37

来源：

BIOTECHNOLOGY LETTERS | 1992年 / 14卷 / 04期

关键词：

D O I：

10.1007/BF01022319

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

During subculturing of recombinant Escherichia coli under conditions blocking cloned gene expression the plasmid was structurally stable for 200 generations. However, under conditions permitting foreign gene expression only 10% of plasmid containing cells had this gene functional after 100 generations.

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页码：251 / 256

页数：6

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