PCR PROBES FOR CHROMOSOME IN-SITU HYBRIDIZATION OF LARGE-INSERT BACTERIAL RECOMBINANTS

被引：18

作者：

KROISEL, PM

IOANNOU, PA

DEJONG, PJ

机构：

[1] LAWRENCE LIVERMORE NATL LAB,CTR HUMAN GENOME,LIVERMORE,CA 94550

[2] CYPRUS INST NEUROL & GENET,NICOSIA,CYPRUS

来源：

CYTOGENETICS AND CELL GENETICS | 1994年 / 65卷 / 1-2期

关键词：

D O I：

10.1159/000133609

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have developed a procedure for efficient in situ hybridization of bacterial recombinants created with various types of large-insert cloning vectors. Minimal quantities of crude DNA are amplified and labeled during the degenerate-oligonucleotide-primed polymerase chain reaction. The resulting probes generate high-intensity fluorescent hybridization signals on metaphase chromosomes and on interphase nuclei.

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页码：97 / 100

页数：4

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