DIAGNOSIS OF LEGIONELLA-PNEUMOPHILA INFECTION BY POLYMERASE CHAIN-REACTION

被引：30

作者：

KOIDE, M

SAITO, A

机构：

[1] UNIV RYUKYUS,FAC MED,DEPT INTERNAL MED 1,OKINAWA 90301,JAPAN

[2] UNIV RYUKYUS,FAC MED,COMPREHENS MED RES INST,OKINAWA 90301,JAPAN

来源：

CLINICAL INFECTIOUS DISEASES | 1995年 / 21卷 / 01期

关键词：

D O I：

10.1093/clinids/21.1.199

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We examined the application of the polymerase chain reaction (PCR) for the diagnosis of legionellosis. Eight intratracheal aspirates were collected from a patient with pneumonia caused by Legionella pneumophila serogroup 2, and serial 10-fold dilutions of the samples were obtained. Two samples were positive for L. pneumophila by the direct fluorescent antibody (DFA) method down to a 10(-2) concentration, and one was positive down to a 10(-4) concentration. PCR was positive for all eight samples, and the sensitivity was greater than that of the DFA method. Only one of the eight samples yielded organisms in culture: the L. pneumophila serogroup 2 strain was isolated on buffered charcoal yeast extract alpha agar as an atypical white, papillate colony.

引用

页码：199 / 201

页数：3

共 8 条

[1] DNA-SEQUENCE OF MIP, A LEGIONELLA-PNEUMOPHILA GENE ASSOCIATED WITH MACROPHAGE INFECTIVITY [J].