THE MECHANISMS CONTROLLING RIBOSOMAL-PROTEIN L1 PRE-MESSENGER-RNA SPLICING ARE MAINTAINED IN EVOLUTION AND RELY ON CONSERVED INTRON SEQUENCES

被引:14
作者
PRISLEI, S
SPERANDIO, S
FRAGAPANE, P
CAFFARELLI, E
PRESUTTI, C
BOZZONI, I
机构
[1] CNR,CTR ACIDI NUCL,ROME,ITALY
[2] UNIV ROME LA SAPIENZA,IST PASTEUR FDN CENCI BOLOGNETTI,DIPARTIMENTO GENET & BIOL MOLEC,I-00185 ROME,ITALY
关键词
D O I
10.1093/nar/20.17.4473
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Sequences corresponding to the third intron of the X.laevis L1 ribosomal protein gene were isolated from the second copy of the X.laevis gene and from the single copy of X.tropicalis. Sequence comparison revealed that the three introns share an unusual sequence conservation which spans a region of 110 nucleotides. In addition, they have the same suboptimal 5' splice sites. The three introns show similar features upon oocyte microinjection: they have very low splicing efficiency and undergo the same site specific cleavages which lead to the accumulation of truncated molecules. Computer analysis and RNAse digestions have allowed to assigne to the conserved region a specific secondary structure. Mutational analysis has shown that this structure is important for conferring the cleavage phenotype to these three introns. Competition experiments show that the cleavage phenotype can be prevented by coinjection of excess amounts of homologous sequences.
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页码:4473 / 4479
页数:7
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