HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY (HPLC) - AN IMPORTANT TECHNIQUE IN THE STUDIES OF LIGNOCAINE AND ITS METABOLISM

The high performance liquid chromatography (HPLC) assays which have been developed for the studies of lignocaine over the past 15 years have been reviewed. Studies of lignocaine and its metabolism have been benefited greatly by utilizing this modern technique. The sensitivity and process time of BPLC have been greatly improved by modifications including the use of solid-phase extraction for sample preparation. A further modification in the assay of hydroxylated derivatives is presented. In the study of lignocaine-monoetnylglycine-xylidide (MEGX) as a dynamic test of liver function, HPLC assay possesses some advantages over fluorescence polarisation immunoassay (FPIA).