DYE-MEDIATED PHOTOLYSIS OF NORMAL AND NEOPLASTIC HEMATOPOIETIC-CELLS

被引：75

作者：

SIEBER, F ^{[1
]}

STUART, RK ^{[1
]}

ROWLEY, SD ^{[1
]}

SHARKIS, SJ ^{[1
]}

SENSENBRENNER, LL ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV HOSP, CTR ONCOL, BALTIMORE, MD 21205 USA

来源：

LEUKEMIA RESEARCH | 1987年 / 11卷 / 01期

关键词：

D O I：

10.1016/0145-2126(87)90104-4

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The purpose of this study was to determine the sensitivity to merocyanine 540 (MC 540)-mediated photolysis of normal human hematopoietic progenitor cells and four leukemia cell lines (Daudi, Raji, K562 and HL-60). Late erythroid progenitors were the most sensitive normal cells. Early erythroid progenitors were of intermediate sensitivity. Granulocyte/macrophage progenitors and multipotent progenitors were the least sensitive normal marrow cells. A combination of dye concentration, serum concentration, and illumination that eliminated 50% of multipotent progenitor cells reduced the concentration of leukemic cells by .gtoreq. 4.5 log. It is conceivable that this difference in photosensitivity can be exploited for the extracorporeal purging of autologous remission marrow grafts.

引用

页码：43 / 49

页数：7