ELECTROKINETIC ISOLATION OF VESICLES AND RIBOSOMES DERIVED FROM SERRATIA-MARCESCENS

被引：7

作者：

HAWKER, DTL ^{[1
]}

TODD, P ^{[1
]}

DAVIS, RH ^{[1
]}

LAWSON, RC ^{[1
]}

RUDGE, SR ^{[1
]}

机构：

[1] UNIV COLORADO,DEPT CHEM ENGN,BOULDER,CO 80309

来源：

BIOTECHNOLOGY PROGRESS | 1992年 / 8卷 / 05期

关键词：

D O I：

10.1021/bp00017a010

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Ribosomes and vesicles derived from the bacterium Serratia marcescens were separated from each other and from solubles using density gradient electrophoresis. Transport relationships were used to determine the electrophoretic mobilities of the particles. The effects of convection, sedimentation and diffusion were found to be negligible. The electrophoretic mobility obtained for the ribosome peak is -7 x 10(-5) cm2/(V.S). Under appropriate conditions, two vesicle peaks were obtained, the first with a mobility of -4 x 10(-5) cm2/(V-s) and the second with -9 x 10(-6) cm2/(V.s). This information can be used to predict the resolution of the separands in large-scale electrophoretic separations.

引用

页码：429 / 435

页数：7

共 29 条

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[2]

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[3]

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